A User’s Guide to Cell-Free Protein Synthesis

Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more. The advantages of CFPS over in vivo protein expression include its open system, the elimination of reliance on livi...

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Published inMethods and protocols Vol. 2; no. 1; p. 24
Main Authors Gregorio, Nicole E., Levine, Max Z., Oza, Javin P.
Format Journal Article
LanguageEnglish
Published Switzerland MDPI 12.03.2019
MDPI AG
Subjects
cell-free metabolic engineering (CFME)
cell-free protein expression (CFPE)
cell-free protein synthesis (CFPS)
cell-free synthetic biology
in vitro protein synthesis
in vitro transcription-translation (TX-TL)
Review
in vitro protein synthesis
in vitro transcription-translation (TX-TL)
cell-free protein synthesis (CFPS)
cell-free synthetic biology
cell-free metabolic engineering (CFME)
cell-free protein expression (CFPE)
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Abstract Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more. The advantages of CFPS over in vivo protein expression include its open system, the elimination of reliance on living cells, and the ability to focus all system energy on production of the protein of interest. Over the last 60 years, the CFPS platform has grown and diversified greatly, and it continues to evolve today. Both new applications and new types of extracts based on a variety of organisms are current areas of development. However, new users interested in CFPS may find it challenging to implement a cell-free platform in their laboratory due to the technical and functional considerations involved in choosing and executing a platform that best suits their needs. Here we hope to reduce this barrier to implementing CFPS by clarifying the similarities and differences amongst cell-free platforms, highlighting the various applications that have been accomplished in each of them, and detailing the main methodological and instrumental requirement for their preparation. Additionally, this review will help to contextualize the landscape of work that has been done using CFPS and showcase the diversity of applications that it enables.
AbstractList Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more. The advantages of CFPS over in vivo protein expression include its open system, the elimination of reliance on living cells, and the ability to focus all system energy on production of the protein of interest. Over the last 60 years, the CFPS platform has grown and diversified greatly, and it continues to evolve today. Both new applications and new types of extracts based on a variety of organisms are current areas of development. However, new users interested in CFPS may find it challenging to implement a cell-free platform in their laboratory due to the technical and functional considerations involved in choosing and executing a platform that best suits their needs. Here we hope to reduce this barrier to implementing CFPS by clarifying the similarities and differences amongst cell-free platforms, highlighting the various applications that have been accomplished in each of them, and detailing the main methodological and instrumental requirement for their preparation. Additionally, this review will help to contextualize the landscape of work that has been done using CFPS and showcase the diversity of applications that it enables.
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more. The advantages of CFPS over in vivo protein expression include its open system, the elimination of reliance on living cells, and the ability to focus all system energy on production of the protein of interest. Over the last 60 years, the CFPS platform has grown and diversified greatly, and it continues to evolve today. Both new applications and new types of extracts based on a variety of organisms are current areas of development. However, new users interested in CFPS may find it challenging to implement a cell-free platform in their laboratory due to the technical and functional considerations involved in choosing and executing a platform that best suits their needs. Here we hope to reduce this barrier to implementing CFPS by clarifying the similarities and differences amongst cell-free platforms, highlighting the various applications that have been accomplished in each of them, and detailing the main methodological and instrumental requirement for their preparation. Additionally, this review will help to contextualize the landscape of work that has been done using CFPS and showcase the diversity of applications that it enables.Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more. The advantages of CFPS over in vivo protein expression include its open system, the elimination of reliance on living cells, and the ability to focus all system energy on production of the protein of interest. Over the last 60 years, the CFPS platform has grown and diversified greatly, and it continues to evolve today. Both new applications and new types of extracts based on a variety of organisms are current areas of development. However, new users interested in CFPS may find it challenging to implement a cell-free platform in their laboratory due to the technical and functional considerations involved in choosing and executing a platform that best suits their needs. Here we hope to reduce this barrier to implementing CFPS by clarifying the similarities and differences amongst cell-free platforms, highlighting the various applications that have been accomplished in each of them, and detailing the main methodological and instrumental requirement for their preparation. Additionally, this review will help to contextualize the landscape of work that has been done using CFPS and showcase the diversity of applications that it enables.
Author Gregorio, Nicole E.
Levine, Max Z.
Oza, Javin P.
AuthorAffiliation 2 Department of Chemistry and Biochemistry, California Polytechnic State University, San Luis Obispo, CA 93407, USA
3 Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, CA 93407, USA
1 Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, CA 93407, USA; negregor@calpoly.edu (N.E.G.); mzlevine@calpoly.edu (M.Z.L.)
AuthorAffiliation_xml – name: 3 Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, CA 93407, USA
– name: 1 Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, CA 93407, USA; negregor@calpoly.edu (N.E.G.); mzlevine@calpoly.edu (M.Z.L.)
– name: 2 Department of Chemistry and Biochemistry, California Polytechnic State University, San Luis Obispo, CA 93407, USA
Author_xml – sequence: 1
  givenname: Nicole E.
  orcidid: 0000-0002-7287-209X
  surname: Gregorio
  fullname: Gregorio, Nicole E.
– sequence: 2
  givenname: Max Z.
  orcidid: 0000-0001-8896-6632
  surname: Levine
  fullname: Levine, Max Z.
– sequence: 3
  givenname: Javin P.
  orcidid: 0000-0002-2902-6939
  surname: Oza
  fullname: Oza, Javin P.
BackLink https://www.ncbi.nlm.nih.gov/pubmed/31164605$$D View this record in MEDLINE/PubMed
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Issue 1
Keywords in vitro protein synthesis
in vitro transcription-translation (TX-TL)
cell-free protein synthesis (CFPS)
cell-free synthetic biology
cell-free metabolic engineering (CFME)
cell-free protein expression (CFPE)
Language English
License https://creativecommons.org/licenses/by/4.0
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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Snippet Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic...
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SubjectTerms cell-free metabolic engineering (CFME)
cell-free protein expression (CFPE)
cell-free protein synthesis (CFPS)
cell-free synthetic biology
in vitro protein synthesis
in vitro transcription-translation (TX-TL)
Review
Title A User’s Guide to Cell-Free Protein Synthesis
URI https://www.ncbi.nlm.nih.gov/pubmed/31164605
https://www.proquest.com/docview/2340037574
https://pubmed.ncbi.nlm.nih.gov/PMC6481089
https://doaj.org/article/b3e4500eec584c5b95b824cf7c529d23
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