Green tea component epigallocatechin-3-gallate decreases expression of osteopontin via a decrease in mRNA half-life in cell lines of metastatic hepatocellular carcinoma

• Published in: Surgery Vol. 158; no. 4; pp. 1039 - 1048
• Main Authors: Zapf, Matthew A.C., BA, Kothari, Anai N., MD, Weber, Cynthia E., MD, Arffa, Matthew L., BA, Wai, Phillip Y., MD, Driver, Joseph, BS, Gupta, Gopal N., MD, Kuo, Paul C., MD, MS, MBA, FACS, Mi, Zhiyong, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2015
• Subjects: Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Carcinoma, Hepatocellular - drug therapy; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Catechin - analogs & derivatives; Catechin - pharmacology; Catechin - therapeutic use; Cell Line, Tumor; Cell Movement - drug effects; Enzyme-Linked Immunosorbent Assay; Half-Life; Humans; Liver Neoplasms - drug therapy; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Neoplasm Metastasis; Osteopontin - genetics; Osteopontin - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Surgery

Introduction Osteopontin (OPN) mediates metastasis and invasion of hepatocellular carcinoma (HCC). Epigallocatechin-3-gallate (EGCG), found in green tea, suppresses HCC tumor growth in vitro. We sought to investigate the role of EGCG in modulating OPN in cell lines of metastatic HCC. Methods Experim...