Epidermal growth factor induces a trophectoderm lineage transcriptome resembling that of human embryos during reconstruction of blastoids from extended pluripotent stem cells

• Published in: Cell proliferation Vol. 55; no. 11; pp. e13317 - n/a
• Main Authors: Zhang, Yingying, An, Chenrui, Yu, Yanhong, Lin, Jiajing, Jin, Long, Li, Chaohui, Tan, Tao, Yu, Yang, Fan, Yong
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.11.2022; John Wiley and Sons Inc
• Subjects: Analysis; Antibodies; Blastocyst - metabolism; Blastocysts; Cell culture; Cell differentiation; Cell Lineage; Data analysis; Embryo, Mammalian; Embryogenesis; Embryonic growth stage; Embryos; Epidermal growth factor; Epidermal Growth Factor - metabolism; Epidermal Growth Factor - pharmacology; Epigenetics; Female; Gene expression; Gene sequencing; Genomic imprinting; Growth factors; Humans; Humidity; Immunofluorescence; Medical research; Optimization; Original; Pluripotency; Pluripotent Stem Cells; Pregnancy; Reconstruction; Ribonucleic acid; RNA; RNA sequencing; Signal transduction; Signaling; Stem cells; Transcriptome; Transcriptomes; Trophectoderm; Japan

Objectives This study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)‐like cell induction with addition of EGF and improve the quality of the reconstructing blastoids. Materials and Methods TE‐like cells were differentiated from human EPSCs. RNA‐seq data analys...