Epidermal growth factor induces a trophectoderm lineage transcriptome resembling that of human embryos during reconstruction of blastoids from extended pluripotent stem cells

Objectives This study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)‐like cell induction with addition of EGF and improve the quality of the reconstructing blastoids. Materials and Methods TE‐like cells were differentiated from human EPSCs. RNA‐seq data analys...

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Published inCell proliferation Vol. 55; no. 11; pp. e13317 - n/a
Main Authors Zhang, Yingying, An, Chenrui, Yu, Yanhong, Lin, Jiajing, Jin, Long, Li, Chaohui, Tan, Tao, Yu, Yang, Fan, Yong
Format Journal Article
LanguageEnglish
Published England John Wiley & Sons, Inc 01.11.2022
John Wiley and Sons Inc
Subjects
Analysis
Antibodies
Blastocyst - metabolism
Blastocysts
Cell culture
Cell differentiation
Cell Lineage
Data analysis
Embryo, Mammalian
Embryogenesis
Embryonic growth stage
Embryos
Epidermal growth factor
Epidermal Growth Factor - metabolism
Epidermal Growth Factor - pharmacology
Epigenetics
Female
Gene expression
Gene sequencing
Genomic imprinting
Growth factors
Humans
Humidity
Immunofluorescence
Medical research
Optimization
Original
Pluripotency
Pluripotent Stem Cells
Pregnancy
Reconstruction
Ribonucleic acid
RNA
RNA sequencing
Signal transduction
Signaling
Stem cells
Transcriptome
Transcriptomes
Trophectoderm
Japan
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Abstract Objectives This study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)‐like cell induction with addition of EGF and improve the quality of the reconstructing blastoids. Materials and Methods TE‐like cells were differentiated from human EPSCs. RNA‐seq data analysis was performed to compare with TE‐like cells from multiple human pluripotent stem cells (hPSCs) and embryos. A small‐scale compound selection was performed for optimizing the TE‐like cell induction and the efficiency was characterized using TE‐lineage markers expression by immunofluorescence stanning. Blastoids were generated by using the optimized TE‐like cells and the undifferentiated human EPSCs through three‐dimensional culture system. Single‐cell RNA sequencing was performed to investigate the lineage segregation of the optimized blastoids to human blastocysts. Results TE‐like cells derived from human EPSCs exhibited similar transcriptome with TE cells from embryos. Additionally, TE‐like cells from multiple naive hPSCs exhibited heterogeneous gene expression patterns and signalling pathways because of the incomplete silencing of naive‐specific genes and loss of imprinting. Furthermore, with the addition of EGF, TE‐like cells derived from human EPSCs enhanced the TE lineage‐related signalling pathways and exhibited more similar transcriptome to human embryos. Through resembling with undifferentiated human EPSCs, we elevated the quality and efficiency of reconstructing blastoids and separated more lineage cells with precise temporal and spatial expression, especially the PE lineage. Conclusion Addition of EGF enhanced TE lineage differentiation and human blastoids reconstruction. The optimized blastoids could be used as a blastocyst model for simulating early embryonic development. Trophectoderm (TE)‐like cells derived from human extended pluripotent stem cells (EPSCs) form human preimplantation TE by precisely upregulating the expression of multiple TE‐specific marker genes and maintaining gene imprinting. Addition of epidermal growth factor (EGF) promoted TE lineage‐related signalling pathways and increased the efficiency of blastoid generation. Single‐cell transcriptomics showed enhanced lineage segregation of the optimized blastoids to human blastocysts, especially the primitive endoderm, with high fidelity.
AbstractList This study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)-like cell induction with addition of EGF and improve the quality of the reconstructing blastoids. TE-like cells were differentiated from human EPSCs. RNA-seq data analysis was performed to compare with TE-like cells from multiple human pluripotent stem cells (hPSCs) and embryos. A small-scale compound selection was performed for optimizing the TE-like cell induction and the efficiency was characterized using TE-lineage markers expression by immunofluorescence stanning. Blastoids were generated by using the optimized TE-like cells and the undifferentiated human EPSCs through three-dimensional culture system. Single-cell RNA sequencing was performed to investigate the lineage segregation of the optimized blastoids to human blastocysts. TE-like cells derived from human EPSCs exhibited similar transcriptome with TE cells from embryos. Additionally, TE-like cells from multiple naive hPSCs exhibited heterogeneous gene expression patterns and signalling pathways because of the incomplete silencing of naive-specific genes and loss of imprinting. Furthermore, with the addition of EGF, TE-like cells derived from human EPSCs enhanced the TE lineage-related signalling pathways and exhibited more similar transcriptome to human embryos. Through resembling with undifferentiated human EPSCs, we elevated the quality and efficiency of reconstructing blastoids and separated more lineage cells with precise temporal and spatial expression, especially the PE lineage. Addition of EGF enhanced TE lineage differentiation and human blastoids reconstruction. The optimized blastoids could be used as a blastocyst model for simulating early embryonic development.
Trophectoderm (TE)‐like cells derived from human extended pluripotent stem cells (EPSCs) form human preimplantation TE by precisely upregulating the expression of multiple TE‐specific marker genes and maintaining gene imprinting. Addition of epidermal growth factor (EGF) promoted TE lineage‐related signalling pathways and increased the efficiency of blastoid generation. Single‐cell transcriptomics showed enhanced lineage segregation of the optimized blastoids to human blastocysts, especially the primitive endoderm, with high fidelity.
This study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)-like cell induction with addition of EGF and improve the quality of the reconstructing blastoids.OBJECTIVESThis study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)-like cell induction with addition of EGF and improve the quality of the reconstructing blastoids.TE-like cells were differentiated from human EPSCs. RNA-seq data analysis was performed to compare with TE-like cells from multiple human pluripotent stem cells (hPSCs) and embryos. A small-scale compound selection was performed for optimizing the TE-like cell induction and the efficiency was characterized using TE-lineage markers expression by immunofluorescence stanning. Blastoids were generated by using the optimized TE-like cells and the undifferentiated human EPSCs through three-dimensional culture system. Single-cell RNA sequencing was performed to investigate the lineage segregation of the optimized blastoids to human blastocysts.MATERIALS AND METHODSTE-like cells were differentiated from human EPSCs. RNA-seq data analysis was performed to compare with TE-like cells from multiple human pluripotent stem cells (hPSCs) and embryos. A small-scale compound selection was performed for optimizing the TE-like cell induction and the efficiency was characterized using TE-lineage markers expression by immunofluorescence stanning. Blastoids were generated by using the optimized TE-like cells and the undifferentiated human EPSCs through three-dimensional culture system. Single-cell RNA sequencing was performed to investigate the lineage segregation of the optimized blastoids to human blastocysts.TE-like cells derived from human EPSCs exhibited similar transcriptome with TE cells from embryos. Additionally, TE-like cells from multiple naive hPSCs exhibited heterogeneous gene expression patterns and signalling pathways because of the incomplete silencing of naive-specific genes and loss of imprinting. Furthermore, with the addition of EGF, TE-like cells derived from human EPSCs enhanced the TE lineage-related signalling pathways and exhibited more similar transcriptome to human embryos. Through resembling with undifferentiated human EPSCs, we elevated the quality and efficiency of reconstructing blastoids and separated more lineage cells with precise temporal and spatial expression, especially the PE lineage.RESULTSTE-like cells derived from human EPSCs exhibited similar transcriptome with TE cells from embryos. Additionally, TE-like cells from multiple naive hPSCs exhibited heterogeneous gene expression patterns and signalling pathways because of the incomplete silencing of naive-specific genes and loss of imprinting. Furthermore, with the addition of EGF, TE-like cells derived from human EPSCs enhanced the TE lineage-related signalling pathways and exhibited more similar transcriptome to human embryos. Through resembling with undifferentiated human EPSCs, we elevated the quality and efficiency of reconstructing blastoids and separated more lineage cells with precise temporal and spatial expression, especially the PE lineage.Addition of EGF enhanced TE lineage differentiation and human blastoids reconstruction. The optimized blastoids could be used as a blastocyst model for simulating early embryonic development.CONCLUSIONAddition of EGF enhanced TE lineage differentiation and human blastoids reconstruction. The optimized blastoids could be used as a blastocyst model for simulating early embryonic development.
ObjectivesThis study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)‐like cell induction with addition of EGF and improve the quality of the reconstructing blastoids.Materials and MethodsTE‐like cells were differentiated from human EPSCs. RNA‐seq data analysis was performed to compare with TE‐like cells from multiple human pluripotent stem cells (hPSCs) and embryos. A small‐scale compound selection was performed for optimizing the TE‐like cell induction and the efficiency was characterized using TE‐lineage markers expression by immunofluorescence stanning. Blastoids were generated by using the optimized TE‐like cells and the undifferentiated human EPSCs through three‐dimensional culture system. Single‐cell RNA sequencing was performed to investigate the lineage segregation of the optimized blastoids to human blastocysts.ResultsTE‐like cells derived from human EPSCs exhibited similar transcriptome with TE cells from embryos. Additionally, TE‐like cells from multiple naive hPSCs exhibited heterogeneous gene expression patterns and signalling pathways because of the incomplete silencing of naive‐specific genes and loss of imprinting. Furthermore, with the addition of EGF, TE‐like cells derived from human EPSCs enhanced the TE lineage‐related signalling pathways and exhibited more similar transcriptome to human embryos. Through resembling with undifferentiated human EPSCs, we elevated the quality and efficiency of reconstructing blastoids and separated more lineage cells with precise temporal and spatial expression, especially the PE lineage.ConclusionAddition of EGF enhanced TE lineage differentiation and human blastoids reconstruction. The optimized blastoids could be used as a blastocyst model for simulating early embryonic development.
Objectives: This study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)-like cell induction with addition of EGF and improve the quality of the reconstructing blastoids. Materials and Methods: TE-like cells were differentiated from human EPSCs. RNA-seq data analysis was performed to compare with TE-like cells from multiple human pluripotent stem cells (hPSCs) and embryos. A small-scale compound selection was performed for optimizing the TE-like cell induction and the efficiency was characterized using TE-lineage markers expression by immunofluorescence stanning. Blastoids were generated by using the optimized TE-like cells and the undifferentiated human EPSCs through three-dimensional culture system. Single-cell RNA sequencing was performed to investigate the lineage segregation of the optimized blastoids to human blastocysts. Results: TE-like cells derived from human EPSCs exhibited similar transcriptome with TE cells from embryos. Additionally, TE-like cells from multiple naive hPSCs exhibited heterogeneous gene expression patterns and signalling pathways because of the incomplete silencing of naive-specific genes and loss of imprinting. Furthermore, with the addition of EGF, TE-like cells derived from human EPSCs enhanced the TE lineage-related signalling pathways and exhibited more similar transcriptome to human embryos. Through resembling with undifferentiated human EPSCs, we elevated the quality and efficiency of reconstructing blastoids and separated more lineage cells with precise temporal and spatial expression, especially the PE lineage. Conclusion: Addition of EGF enhanced TE lineage differentiation and human blastoids reconstruction. The optimized blastoids could be used as a blastocyst model for simulating early embryonic development.
Objectives This study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)‐like cell induction with addition of EGF and improve the quality of the reconstructing blastoids. Materials and Methods TE‐like cells were differentiated from human EPSCs. RNA‐seq data analysis was performed to compare with TE‐like cells from multiple human pluripotent stem cells (hPSCs) and embryos. A small‐scale compound selection was performed for optimizing the TE‐like cell induction and the efficiency was characterized using TE‐lineage markers expression by immunofluorescence stanning. Blastoids were generated by using the optimized TE‐like cells and the undifferentiated human EPSCs through three‐dimensional culture system. Single‐cell RNA sequencing was performed to investigate the lineage segregation of the optimized blastoids to human blastocysts. Results TE‐like cells derived from human EPSCs exhibited similar transcriptome with TE cells from embryos. Additionally, TE‐like cells from multiple naive hPSCs exhibited heterogeneous gene expression patterns and signalling pathways because of the incomplete silencing of naive‐specific genes and loss of imprinting. Furthermore, with the addition of EGF, TE‐like cells derived from human EPSCs enhanced the TE lineage‐related signalling pathways and exhibited more similar transcriptome to human embryos. Through resembling with undifferentiated human EPSCs, we elevated the quality and efficiency of reconstructing blastoids and separated more lineage cells with precise temporal and spatial expression, especially the PE lineage. Conclusion Addition of EGF enhanced TE lineage differentiation and human blastoids reconstruction. The optimized blastoids could be used as a blastocyst model for simulating early embryonic development. Trophectoderm (TE)‐like cells derived from human extended pluripotent stem cells (EPSCs) form human preimplantation TE by precisely upregulating the expression of multiple TE‐specific marker genes and maintaining gene imprinting. Addition of epidermal growth factor (EGF) promoted TE lineage‐related signalling pathways and increased the efficiency of blastoid generation. Single‐cell transcriptomics showed enhanced lineage segregation of the optimized blastoids to human blastocysts, especially the primitive endoderm, with high fidelity.
This study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)-like cell induction with addition of EGF and improve the quality of the reconstructing blastoids. TE-like cells were differentiated from human EPSCs. RNA-seq data analysis was performed to compare with TE-like cells from multiple human pluripotent stem cells (hPSCs) and embryos. A small-scale compound selection was performed for optimizing the TE-like cell induction and the efficiency was characterized using TE-lineage markers expression by immunofluorescence stanning. Blastoids were generated by using the optimized TE-like cells and the undifferentiated human EPSCs through three-dimensional culture system. Single-cell RNA sequencing was performed to investigate the lineage segregation of the optimized blastoids to human blastocysts. TE-like cells derived from human EPSCs exhibited similar transcriptome with TE cells from embryos. Additionally, TE-like cells from multiple naive hPSCs exhibited heterogeneous gene expression patterns and signalling pathways because of the incomplete silencing of naive-specific genes and loss of imprinting. Furthermore, with the addition of EGF, TE-like cells derived from human EPSCs enhanced the TE lineage-related signalling pathways and exhibited more similar transcriptome to human embryos. Through resembling with undifferentiated human EPSCs, we elevated the quality and efficiency of reconstructing blastoids and separated more lineage cells with precise temporal and spatial expression, especially the PE lineage. Addition of EGF enhanced TE lineage differentiation and human blastoids reconstruction. The optimized blastoids could be used as a blastocyst model for simulating early embryonic development.
Audience Academic
Author Lin, Jiajing
An, Chenrui
Jin, Long
Yu, Yang
Li, Chaohui
Zhang, Yingying
Yu, Yanhong
Tan, Tao
Fan, Yong
AuthorAffiliation 2 Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming China
3 Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Department of Obstetrics and Gynecology Peking University Third Hospital Beijing China
1 Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes The Third Affiliated Hospital of Guangzhou Medical University Guangzhou Guangdong China
AuthorAffiliation_xml – name: 1 Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes The Third Affiliated Hospital of Guangzhou Medical University Guangzhou Guangdong China
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/35880490$$D View this record in MEDLINE/PubMed
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Cites_doi 10.1016/j.celrep.2022.110570
10.1007/s10815-020-01925-0
10.1016/j.cell.2019.09.029
10.1038/s41586-020-2759-x
10.1073/pnas.1319738111
10.1038/s41586-021-04267-8
10.1016/j.stem.2020.06.002
10.1016/j.cell.2005.08.040
10.1038/s41467-021-25853-4
10.1016/j.stem.2014.07.002
10.1038/nature17948
10.1038/labinvest.3780362
10.1038/nsmb.2660
10.1016/j.stem.2021.02.025
10.1073/pnas.1303094110
10.1016/j.fertnstert.2020.01.009
10.1016/j.cell.2014.08.029
10.1016/j.stem.2013.11.015
10.1530/REP-09-0447
10.1038/nbt.2239
10.1242/dev.145318
10.1016/j.stem.2016.01.019
10.1038/nbt761
10.1038/s41586-021-03635-8
10.1038/s41586-020-2734-6
10.1177/43.6.7539466
10.4049/jimmunol.0803769
10.1371/journal.pone.0234628
10.1016/j.stem.2016.06.011
10.1095/biolreprod.107.066480
10.1242/dev.146811
10.1038/s41586-018-0051-0
10.1038/35047554
10.1016/j.stem.2021.04.031
10.1038/s41586-021-03372-y
10.1016/j.stemcr.2016.02.005
10.1016/j.ymthe.2018.07.009
10.1038/s41421-021-00316-8
10.1038/nature12745
10.1016/j.cell.2016.03.023
10.1038/s41467-017-00308-x
10.1016/j.stem.2021.03.013
10.1016/j.stem.2009.05.015
10.1016/j.devcel.2014.06.019
10.1186/s12964-021-00783-2
10.1016/j.celrep.2018.12.099
10.1038/s41467-021-23320-8
10.1016/j.stemcr.2019.10.009
10.1016/j.ydbio.2005.12.033
10.1016/j.celrep.2020.108419
10.1016/j.bbamcr.2020.118736
10.1093/humrep/der075
10.1002/stem.2261
10.1095/biolreprod.112.099937
10.1038/icb.2017.45
10.1007/s10561-014-9454-9
10.1038/cdd.2008.73
10.1071/RD9951465
10.1016/S1472-6483(10)60649-9
10.1016/j.isci.2018.12.014
10.1016/j.stemcr.2016.01.006
10.1093/humupd/dml048
10.7554/eLife.52504
10.1038/nature19096
10.1016/j.stemcr.2021.09.002
10.1016/j.cell.2017.02.005
10.1073/pnas.1604747113
10.1038/nature24052
10.1016/j.stem.2017.11.004
10.1038/nri1897
10.1038/s41586-019-1500-0
10.1016/j.cell.2019.01.043
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2022 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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Notes Funding information
Guangdong Basic and Applied Basic Research Foundation, Grant/Award Number: 2021B1515020069; Guangzhou Education Bureau Basic Research Foundation, Grant/Award Number: 202032769; National Key Research and Development Program of China, Grant/Award Numbers: 2019YFA0110804, 2021YFC2700303; National Natural Science Foundation of China, Grant/Award Numbers: 81871162, 82071723
Yingying Zhang and Chenrui An contributed equally to this research.
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Funding information Guangdong Basic and Applied Basic Research Foundation, Grant/Award Number: 2021B1515020069; Guangzhou Education Bureau Basic Research Foundation, Grant/Award Number: 202032769; National Key Research and Development Program of China, Grant/Award Numbers: 2019YFA0110804, 2021YFC2700303; National Natural Science Foundation of China, Grant/Award Numbers: 81871162, 82071723
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0000-0002-7107-773X
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References 2017; 8
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2006; 292
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2013; 13
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2008; 15
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2006; 6
2016; 165
2020; 587
2020; 33
2020; 586
2016; 18
2018; 22
2014; 111
2014; 158
2007; 13
2018; 26
2012; 30
1995; 7
2017; 95
2001; 81
2021; 16
2016; 6
2021; 12
2018; 557
2002; 20
2016; 537
2005; 123
2010; 139
1995; 43
2021; 19
2020; 27
2019; 179
2001; 2
2016; 533
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2009; 4
2017; 144
2014; 30
2012; 87
2019; 572
2019; 176
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References_xml – volume: 19
  start-page: 101
  issue: 1
  year: 2021
  article-title: EGF stimulates human trophoblast cell invasion by downregulating ID3‐mediated KISS1 expression
  publication-title: Cell Commun Signal
– volume: 504
  start-page: 282
  issue: 7479
  year: 2013
  end-page: 286
  article-title: Derivation of novel human ground state naive pluripotent stem cells
  publication-title: Nature
– volume: 26
  start-page: 1440
  issue: 6
  year: 2011
  end-page: 1449
  article-title: Transcriptome analysis reveals dialogues between human trophectoderm and endometrial cells during the implantation period
  publication-title: Hum Reprod
– volume: 6
  start-page: 257
  issue: 2
  year: 2016
  end-page: 272
  article-title: What is trophoblast? A combination of criteria define human first‐trimester trophoblast
  publication-title: Stem Cell Rep
– volume: 169
  start-page: 243
  issue: 2
  year: 2017
  end-page: 257 e25
  article-title: Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency
  publication-title: Cell
– volume: 34
  start-page: 847
  issue: 4
  year: 2016
  end-page: 859
  article-title: A primitive growth factor, NME7(AB), is sufficient to induce stable naive state human pluripotency; reprogramming in this novel growth factor confers superior differentiation
  publication-title: Stem Cells
– volume: 95
  start-page: 695
  issue: 8
  year: 2017
  end-page: 704
  article-title: dNK cells facilitate the interaction between trophoblastic and endothelial cells via VEGF‐C and HGF
  publication-title: Immunol Cell Biol
– volume: 18
  start-page: 323
  issue: 3
  year: 2016
  end-page: 329
  article-title: Naive human pluripotent cells feature a methylation landscape devoid of blastocyst or germline memory
  publication-title: Cell Stem Cell
– volume: 179
  start-page: 687
  issue: 3
  year: 2019
  end-page: 702 e618
  article-title: Generation of blastocyst‐like structures from mouse embryonic and adult cell cultures
  publication-title: Cell
– volume: 13
  start-page: 663
  issue: 6
  year: 2013
  end-page: 675
  article-title: Induction of a human pluripotent state with distinct regulatory circuitry that resembles preimplantation epiblast
  publication-title: Cell Stem Cell
– volume: 1867
  issue: 9
  year: 2020
  article-title: Deciphering a distinct regulatory network of TEAD4, CDX2 and GATA3 in humans for trophoblast transition from embryonic stem cells
  publication-title: Biochim Biophys Acta Mol Cell Res
– volume: 15
  start-page: 1481
  issue: 9
  year: 2008
  end-page: 1490
  article-title: HCCS1 overexpression induces apoptosis via cathepsin D and intracellular calcium, and HCCS1 disruption in mice causes placental abnormality
  publication-title: Cell Death Differ
– volume: 26
  start-page: 815
  issue: 4
  year: 2019
  end-page: 824 e4
  article-title: Transcriptional heterogeneity in naive and primed human pluripotent stem cells at single‐cell resolution
  publication-title: Cell Rep
– volume: 22
  start-page: 50
  issue: 1
  year: 2018
  end-page: 63 e6
  article-title: Derivation of human trophoblast stem cells
  publication-title: Cell Stem Cell
– volume: 37
  start-page: 2699
  issue: 11
  year: 2020
  end-page: 2711
  article-title: The unknown human trophectoderm: implication for biopsy at the blastocyst stage
  publication-title: J Assist Reprod Genet
– volume: 79
  start-page: 58
  issue: 1
  year: 2008
  end-page: 65
  article-title: CX3CL1 and CCL14 regulate extracellular matrix and adhesion molecules in the trophoblast: potential roles in human embryo implantation
  publication-title: Biol Reprod
– volume: 586
  start-page: 101
  issue: 7827
  year: 2020
  end-page: 107
  article-title: Reprogramming roadmap reveals route to human induced trophoblast stem cells
  publication-title: Nature
– volume: 30
  start-page: 531
  issue: 6
  year: 2012
  end-page: 542
  article-title: Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells
  publication-title: Nat Biotechnol
– volume: 292
  start-page: 277
  issue: 2
  year: 2006
  end-page: 289
  article-title: Trophoblast cells exhibit differential responses to laminin isoforms
  publication-title: Dev Biol
– volume: 4
  start-page: 487
  issue: 6
  year: 2009
  end-page: 492
  article-title: Naive and primed pluripotent states
  publication-title: Cell Stem Cell
– volume: 19
  start-page: 502
  issue: 4
  year: 2016
  end-page: 515
  article-title: Molecular criteria for defining the naive human pluripotent state
  publication-title: Cell Stem Cell
– volume: 572
  start-page: 660
  issue: 7771
  year: 2019
  end-page: 664
  article-title: Reconstituting the transcriptome and DNA methylome landscapes of human implantation
  publication-title: Nature
– volume: 139
  start-page: 465
  issue: 2
  year: 2010
  end-page: 478
  article-title: Transforming growth factor beta (TGFB) signaling is activated during porcine implantation: proposed role for latency‐associated peptide interactions with integrins at the conceptus–maternal interface
  publication-title: Reproduction
– volume: 20
  start-page: 1261
  issue: 12
  year: 2002
  end-page: 1264
  article-title: BMP4 initiates human embryonic stem cell differentiation to trophoblast
  publication-title: Nat Biotechnol
– volume: 158
  start-page: 1254
  issue: 6
  year: 2014
  end-page: 1269
  article-title: Resetting transcription factor control circuitry toward ground‐state pluripotency in human
  publication-title: Cell
– volume: 28
  start-page: 1023
  issue: 6
  year: 2021
  end-page: 1039 e13
  article-title: Capturing human trophoblast development with naive pluripotent stem cells in vitro
  publication-title: Cell Stem Cell
– volume: 550
  start-page: 393
  issue: 7676
  year: 2017
  end-page: 397
  article-title: Establishment of mouse expanded potential stem cells
  publication-title: Nature
– volume: 28
  start-page: 1016
  issue: 6
  year: 2021
  end-page: 1022 e4
  article-title: Naive stem cell blastocyst model captures human embryo lineage segregation
  publication-title: Cell Stem Cell
– volume: 113
  start-page: E3882
  issue: 27
  year: 2016
  end-page: E3891
  article-title: Human pluripotent stem cells as a model of trophoblast differentiation in both normal development and disease
  publication-title: Proc Natl Acad Sci U S A
– volume: 2
  start-page: 21
  issue: 1
  year: 2001
  end-page: 32
  article-title: Genomic imprinting: parental influence on the genome
  publication-title: Nat Rev Genet
– volume: 9
  year: 2020
  article-title: Derivation of trophoblast stem cells from naive human pluripotent stem cells
  publication-title: Elife
– volume: 591
  start-page: 627
  issue: 7851
  year: 2021
  article-title: Modelling human blastocysts by reprogramming fibroblasts into iBlastoids
  publication-title: Nature
– volume: 7
  start-page: 81
  issue: 1
  year: 2021
  article-title: Generation of human blastocyst‐like structures from pluripotent stem cells
  publication-title: Cell Discov
– volume: 533
  start-page: 251
  issue: 7602
  year: 2016
  end-page: 254
  article-title: Self‐organization of the in vitro attached human embryo
  publication-title: Nature
– volume: 111
  start-page: 4484
  issue: 12
  year: 2014
  end-page: 4489
  article-title: Derivation of naive human embryonic stem cells
  publication-title: Proc Natl Acad Sci U S A
– volume: 81
  start-page: 1469
  issue: 11
  year: 2001
  end-page: 1483
  article-title: Focal adhesion kinase is a key mediator of human trophoblast development
  publication-title: Lab Invest
– volume: 11
  start-page: 508
  year: 2019
  end-page: 518
  article-title: Derivation of haploid trophoblast stem cells via conversion in vitro
  publication-title: iScience
– volume: 144
  start-page: 876
  issue: 5
  year: 2017
  end-page: 888
  article-title: Genetic redundancy of GATA factors in the extraembryonic trophoblast lineage ensures the progression of preimplantation and postimplantation mammalian development
  publication-title: Development
– volume: 13
  start-page: 121
  issue: 2
  year: 2007
  end-page: 141
  article-title: Molecular circuits shared by placental and cancer cells, and their implications in the proliferative, invasive and migratory capacities of trophoblasts
  publication-title: Hum Reprod Update
– volume: 601
  start-page: 600
  issue: 7894
  year: 2022
  end-page: 605
  article-title: Human blastoids model blastocyst development and implantation
  publication-title: Nature
– volume: 26
  start-page: 2189
  issue: 9
  year: 2018
  end-page: 2205
  article-title: MicroRNA‐218‐5p promotes endovascular trophoblast differentiation and spiral artery remodeling
  publication-title: Mol Ther
– volume: 13
  start-page: 1083
  issue: 6
  year: 2019
  end-page: 1098
  article-title: Wnt inhibition facilitates RNA‐mediated reprogramming of human somatic cells to naive pluripotency
  publication-title: Stem Cell Rep
– volume: 110
  start-page: E1212
  issue: 13
  year: 2013
  end-page: E1221
  article-title: Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4
  publication-title: Proc Natl Acad Sci U S A
– volume: 27
  start-page: 482
  issue: 3
  year: 2020
  end-page: 497 e4
  article-title: Overcoming autocrine FGF signaling‐induced heterogeneity in naive human ESCs enables modeling of random X chromosome inactivation
  publication-title: Cell Stem Cell
– volume: 182
  start-page: 4085
  issue: 7
  year: 2009
  end-page: 4092
  article-title: Vascular endothelial growth factor C facilitates immune tolerance and endovascular activity of human uterine NK cells at the maternal‐fetal interface
  publication-title: J Immunol
– volume: 114
  start-page: 653
  issue: 3
  year: 2020
  end-page: 664 e6
  article-title: Human embryonic stem cell‐derived blastocyst‐like spheroids resemble human trophectoderm during early implantation process
  publication-title: Fertil Steril
– volume: 28
  start-page: 1040
  issue: 6
  year: 2021
  end-page: 1056 e6
  article-title: Human naive epiblast cells possess unrestricted lineage potential
  publication-title: Cell Stem Cell
– volume: 38
  issue: 13
  year: 2022
  article-title: Rif1 and Hmgn3 regulate the conversion of murine trophoblast stem cells
  publication-title: Cell Rep
– volume: 20
  start-page: 1131
  issue: 9
  year: 2013
  end-page: 1139
  article-title: Single‐cell RNA‐Seq profiling of human preimplantation embryos and embryonic stem cells
  publication-title: Nat Struct Mol Biol
– volume: 587
  start-page: 443
  issue: 7834
  year: 2020
  end-page: 447
  article-title: Initiation of a conserved trophectoderm program in human, cow and mouse embryos
  publication-title: Nature
– volume: 15
  start-page: 213
  issue: 2
  year: 2014
  end-page: 225
  article-title: Intact human amniotic membrane differentiated towards the chondrogenic lineage
  publication-title: Cell Tissue Bank
– volume: 557
  start-page: 106
  issue: 7703
  year: 2018
  end-page: 111
  article-title: Blastocyst‐like structures generated solely from stem cells
  publication-title: Nature
– volume: 16
  start-page: 2520
  issue: 10
  year: 2021
  end-page: 2533
  article-title: Large‐scale analysis of imprinting in naive human pluripotent stem cells reveals recurrent aberrations and a potential link to FGF signaling
  publication-title: Stem Cell Rep
– volume: 6
  start-page: 437
  issue: 4
  year: 2016
  end-page: 446
  article-title: Naive pluripotent stem cells derived directly from isolated cells of the human inner cell mass
  publication-title: Stem Cell Rep
– volume: 596
  issue: 7872
  year: 2021
  article-title: Blastocyst‐like structures generated from human pluripotent stem cells (vol 591, pg 620, 2021)
  publication-title: Nature
– volume: 7
  start-page: 1465
  issue: 6
  year: 1995
  end-page: 1470
  article-title: Role of epidermal growth factor (EGF) and its receptor in the development of the human placenta
  publication-title: Reprod Fertil Dev
– volume: 12
  start-page: 5550
  issue: 1
  year: 2021
  article-title: Reconstructing aspects of human embryogenesis with pluripotent stem cells
  publication-title: Nat Commun
– volume: 12
  start-page: 3017
  issue: 1
  year: 2021
  article-title: Chemically defined and xeno‐free culture condition for human extended pluripotent stem cells
  publication-title: Nat Commun
– volume: 8
  start-page: 352
  issue: 1
  year: 2017
  article-title: Decidualisation and placentation defects are a major cause of age‐related reproductive decline
  publication-title: Nat Commun
– volume: 13
  start-page: 591
  issue: 4
  year: 2006
  end-page: 599
  article-title: Defective implantation and placentation: laying the blueprint for pregnancy complications
  publication-title: Reprod Biomed Online
– volume: 176
  start-page: 952
  issue: 5
  year: 2019
  end-page: 965
  article-title: Genomic imprinting and physiological processes in mammals
  publication-title: Cell
– volume: 123
  start-page: 917
  issue: 5
  year: 2005
  end-page: 929
  article-title: Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation
  publication-title: Cell
– volume: 43
  start-page: 579
  issue: 6
  year: 1995
  end-page: 589
  article-title: Differentiation of human trophoblast populations involves alterations in cytokeratin patterns
  publication-title: J Histochem Cytochem
– volume: 87
  start-page: 47
  issue: 2
  year: 2012
  article-title: Role of integrin switch and transforming growth factor Beta 3 in hypoxia‐induced invasion inhibition of human extravillous trophoblast cells
  publication-title: Biol Reprod
– volume: 165
  start-page: 1012
  issue: 4
  year: 2016
  end-page: 1026
  article-title: Single‐cell RNA‐Seq reveals lineage and X chromosome dynamics in human preimplantation embryos
  publication-title: Cell
– volume: 33
  start-page: 108419
  issue: 8
  year: 2020
  article-title: Induction of human trophoblast stem cells from somatic cells and pluripotent stem cells
  publication-title: Cell Rep
– volume: 537
  start-page: 57
  issue: 7618
  year: 2016
  end-page: 62
  article-title: A developmental coordinate of pluripotency among mice, monkeys and humans
  publication-title: Nature
– volume: 6
  start-page: 584
  issue: 8
  year: 2006
  end-page: 594
  article-title: Immunology of placentation in eutherian mammals
  publication-title: Nat Rev Immunol
– volume: 144
  start-page: 2748
  issue: 15
  year: 2017
  end-page: 2763
  article-title: Epigenetic resetting of human pluripotency
  publication-title: Development
– volume: 15
  start-page: 471
  issue: 4
  year: 2014
  end-page: 487
  article-title: Systematic identification of culture conditions for induction and maintenance of naive human pluripotency
  publication-title: Cell Stem Cell
– volume: 30
  start-page: 410
  issue: 4
  year: 2014
  end-page: 422
  article-title: Notch and hippo converge on Cdx2 to specify the trophectoderm lineage in the mouse blastocyst
  publication-title: Dev Cell
– volume: 15
  issue: 6
  year: 2020
  article-title: A lncRNA‐miRNA‐mRNA network for human primed, naive and extended pluripotent stem cells
  publication-title: PLoS One
– ident: e_1_2_9_69_1
  doi: 10.1016/j.celrep.2022.110570
– ident: e_1_2_9_45_1
  doi: 10.1007/s10815-020-01925-0
– ident: e_1_2_9_63_1
  doi: 10.1016/j.cell.2019.09.029
– ident: e_1_2_9_41_1
  doi: 10.1038/s41586-020-2759-x
– ident: e_1_2_9_23_1
  doi: 10.1073/pnas.1319738111
– ident: e_1_2_9_8_1
  doi: 10.1038/s41586-021-04267-8
– ident: e_1_2_9_29_1
  doi: 10.1016/j.stem.2020.06.002
– ident: e_1_2_9_72_1
  doi: 10.1016/j.cell.2005.08.040
– ident: e_1_2_9_4_1
  doi: 10.1038/s41467-021-25853-4
– ident: e_1_2_9_26_1
  doi: 10.1016/j.stem.2014.07.002
– ident: e_1_2_9_38_1
  doi: 10.1038/nature17948
– ident: e_1_2_9_46_1
  doi: 10.1038/labinvest.3780362
– ident: e_1_2_9_64_1
  doi: 10.1038/nsmb.2660
– ident: e_1_2_9_10_1
  doi: 10.1016/j.stem.2021.02.025
– ident: e_1_2_9_19_1
  doi: 10.1073/pnas.1303094110
– ident: e_1_2_9_17_1
  doi: 10.1016/j.fertnstert.2020.01.009
– ident: e_1_2_9_22_1
  doi: 10.1016/j.cell.2014.08.029
– ident: e_1_2_9_24_1
  doi: 10.1016/j.stem.2013.11.015
– ident: e_1_2_9_57_1
  doi: 10.1530/REP-09-0447
– ident: e_1_2_9_16_1
  doi: 10.1038/nbt.2239
– ident: e_1_2_9_43_1
  doi: 10.1242/dev.145318
– ident: e_1_2_9_59_1
  doi: 10.1016/j.stem.2016.01.019
– ident: e_1_2_9_18_1
  doi: 10.1038/nbt761
– ident: e_1_2_9_5_1
  doi: 10.1038/s41586-021-03635-8
– ident: e_1_2_9_31_1
  doi: 10.1038/s41586-020-2734-6
– ident: e_1_2_9_44_1
  doi: 10.1177/43.6.7539466
– ident: e_1_2_9_55_1
  doi: 10.4049/jimmunol.0803769
– ident: e_1_2_9_37_1
  doi: 10.1371/journal.pone.0234628
– ident: e_1_2_9_28_1
  doi: 10.1016/j.stem.2016.06.011
– ident: e_1_2_9_52_1
  doi: 10.1095/biolreprod.107.066480
– ident: e_1_2_9_61_1
  doi: 10.1242/dev.146811
– ident: e_1_2_9_3_1
  doi: 10.1038/s41586-018-0051-0
– ident: e_1_2_9_65_1
  doi: 10.1038/35047554
– ident: e_1_2_9_6_1
  doi: 10.1016/j.stem.2021.04.031
– ident: e_1_2_9_7_1
  doi: 10.1038/s41586-021-03372-y
– ident: e_1_2_9_60_1
  doi: 10.1016/j.stemcr.2016.02.005
– ident: e_1_2_9_50_1
  doi: 10.1016/j.ymthe.2018.07.009
– ident: e_1_2_9_9_1
  doi: 10.1038/s41421-021-00316-8
– ident: e_1_2_9_21_1
  doi: 10.1038/nature12745
– ident: e_1_2_9_39_1
  doi: 10.1016/j.cell.2016.03.023
– ident: e_1_2_9_13_1
  doi: 10.1038/s41467-017-00308-x
– ident: e_1_2_9_30_1
  doi: 10.1016/j.stem.2021.03.013
– ident: e_1_2_9_20_1
  doi: 10.1016/j.stem.2009.05.015
– ident: e_1_2_9_70_1
  doi: 10.1016/j.devcel.2014.06.019
– ident: e_1_2_9_47_1
  doi: 10.1186/s12964-021-00783-2
– ident: e_1_2_9_62_1
  doi: 10.1016/j.celrep.2018.12.099
– ident: e_1_2_9_36_1
  doi: 10.1038/s41467-021-23320-8
– ident: e_1_2_9_27_1
  doi: 10.1016/j.stemcr.2019.10.009
– ident: e_1_2_9_48_1
  doi: 10.1016/j.ydbio.2005.12.033
– ident: e_1_2_9_73_1
  doi: 10.1016/j.celrep.2020.108419
– ident: e_1_2_9_11_1
  doi: 10.1016/j.bbamcr.2020.118736
– ident: e_1_2_9_58_1
  doi: 10.1093/humrep/der075
– ident: e_1_2_9_25_1
  doi: 10.1002/stem.2261
– ident: e_1_2_9_49_1
  doi: 10.1095/biolreprod.112.099937
– ident: e_1_2_9_56_1
  doi: 10.1038/icb.2017.45
– ident: e_1_2_9_53_1
  doi: 10.1007/s10561-014-9454-9
– ident: e_1_2_9_51_1
  doi: 10.1038/cdd.2008.73
– ident: e_1_2_9_67_1
  doi: 10.1071/RD9951465
– ident: e_1_2_9_15_1
  doi: 10.1016/S1472-6483(10)60649-9
– ident: e_1_2_9_71_1
  doi: 10.1016/j.isci.2018.12.014
– ident: e_1_2_9_42_1
  doi: 10.1016/j.stemcr.2016.01.006
– ident: e_1_2_9_68_1
  doi: 10.1093/humupd/dml048
– ident: e_1_2_9_32_1
  doi: 10.7554/eLife.52504
– ident: e_1_2_9_2_1
  doi: 10.1038/nature19096
– ident: e_1_2_9_33_1
  doi: 10.1016/j.stemcr.2021.09.002
– ident: e_1_2_9_34_1
  doi: 10.1016/j.cell.2017.02.005
– ident: e_1_2_9_54_1
  doi: 10.1073/pnas.1604747113
– ident: e_1_2_9_35_1
  doi: 10.1038/nature24052
– ident: e_1_2_9_12_1
  doi: 10.1016/j.stem.2017.11.004
– ident: e_1_2_9_14_1
  doi: 10.1038/nri1897
– ident: e_1_2_9_40_1
  doi: 10.1038/s41586-019-1500-0
– ident: e_1_2_9_66_1
  doi: 10.1016/j.cell.2019.01.043
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Snippet Objectives This study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)‐like cell induction with addition of EGF and...
This study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)-like cell induction with addition of EGF and improve the...
Objectives: This study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)-like cell induction with addition of EGF and...
ObjectivesThis study aims to optimize the human extended pluripotent stem cell (EPSC) to trophectoderm (TE)‐like cell induction with addition of EGF and...
Trophectoderm (TE)‐like cells derived from human extended pluripotent stem cells (EPSCs) form human preimplantation TE by precisely upregulating the expression...
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SourceType Open Access Repository
Aggregation Database
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StartPage e13317
SubjectTerms Analysis
Antibodies
Blastocyst - metabolism
Blastocysts
Cell culture
Cell differentiation
Cell Lineage
Data analysis
Embryo, Mammalian
Embryogenesis
Embryonic growth stage
Embryos
Epidermal growth factor
Epidermal Growth Factor - metabolism
Epidermal Growth Factor - pharmacology
Epigenetics
Female
Gene expression
Gene sequencing
Genomic imprinting
Growth factors
Humans
Humidity
Immunofluorescence
Medical research
Optimization
Original
Pluripotency
Pluripotent Stem Cells
Pregnancy
Reconstruction
Ribonucleic acid
RNA
RNA sequencing
Signal transduction
Signaling
Stem cells
Transcriptome
Transcriptomes
Trophectoderm
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Title Epidermal growth factor induces a trophectoderm lineage transcriptome resembling that of human embryos during reconstruction of blastoids from extended pluripotent stem cells
URI https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fcpr.13317
https://www.ncbi.nlm.nih.gov/pubmed/35880490
https://www.proquest.com/docview/2731031820
https://www.proquest.com/docview/2694962150
https://pubmed.ncbi.nlm.nih.gov/PMC9628219
Volume 55
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