Bistable Epigenetic States Explain Age‐Dependent Decline in Mesenchymal Stem Cell Heterogeneity

• Published in: Stem cells (Dayton, Ohio) Vol. 35; no. 3; pp. 694 - 704
• Main Authors: Hamidouche, Zahia, Rother, Karen, Przybilla, Jens, Krinner, Axel, Clay, Denis, Hopp, Lydia, Fabian, Claire, Stolzing, Alexandra, Binder, Hans, Charbord, Pierre, Galle, Joerg
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01.03.2017; AlphaMed Press; John Wiley and Sons Inc
• Subjects: Age; Aging; Aging - genetics; Animals; Antigens, Ly - metabolism; Bone marrow; Bone Marrow Cells - cytology; Cell Differentiation - genetics; Cell Proliferation; Clone Cells; Computer applications; Epigenesis, Genetic; Epigenetics; FACS; Feedback; Gene Expression Profiling; Genes; Heterogeneity; In vitro methods and tests; Life Sciences; Membrane Proteins - metabolism; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Methylation; Mice, Inbred C57BL; Models, Biological; Models, Genetic; Molecular modelling; Positive feedback; Promoter Regions, Genetic; Regeneration; Replication; Rodents; Stem cell transplantation; Stem cells; Subpopulations; Tissue‐Specific Stem Cells; Transcription; Aging; Epigenetics; FACS; Methylation; Mesenchymal stem cells

The molecular mechanisms by which heterogeneity, a major characteristic of stem cells, is achieved are yet unclear. We here study the expression of the membrane stem cell antigen‐1 (Sca‐1) in mouse bone marrow mesenchymal stem cell (MSC) clones. We show that subpopulations with varying Sca‐1 express...