Pre-staining of glycoprotein in SDS-PAGE by the synthesis of a new hydrazide derivative

• Published in: Proteomics (Weinheim) Vol. 15; no. 21; pp. 3623 - 3628
• Main Authors: Zhou, Ayi, Zhou, Tieli, Yu, Dongdong, Shen, Yingjie, Shen, Jiayi, Zhu, Zhongxin, Jin, Litai, Zhang, Huajie, Wang, Yang
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.11.2015; Wiley Subscription Services, Inc
• Subjects: Avidin - analysis; Chromatography, Liquid; Electrophoresis; Electrophoresis, Gel, Two-Dimensional - methods; Electrophoresis, Polyacrylamide Gel - methods; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Glycoprotein detection; Glycoproteins - analysis; Glycoproteomics; Humans; Hydrazines - chemical synthesis; Hydrazines - chemistry; Orosomucoid - analysis; Pre-stain; Pro-Q Emerald 300; Staining and Labeling - methods; Tandem Mass Spectrometry; Transferrin - analysis; UGF202; Electrophoresis; Pre-stain; Glycoproteomics; Pro-Q Emerald 300; Glycoprotein detection; UGF202

In this study, a new hydrazide derivative (UGF202) was synthesized and introduced as a highly sensitive and selective fluorescent probe to pre‐stain glycoproteins in 1D and 2D SDS‐PAGE. As low as 0.5–1 ng glycoproteins (transferrin, α1‐acid glycoprotein, avidin) could be selectively detected, which...