Ameyamaea chiangmaiensis gen. nov., sp. nov., an acetic acid bacterium in the alpha-Proteobacteria

• Published in: Bioscience, biotechnology, and biochemistry Vol. 73; no. 10; pp. 2156 - 2162
• Main Authors: Yukphan, P.(National Center for Genetic Engineering and Biotechnology, Pathumthani (Thailand)), Malimas, T, Muramatsu, Y, Takahashi, M, Kaneyasu, M, Potacharoen, W, Tanasupawat, S, Nakagawa, Y, Hamana, K, Tahara, Y, Suzuki, K, Tanticharoen M, Yamada, Y
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.10.2009; Japan Society for Bioscience Biotechnology and Agrochemistry
• Subjects: 16S rRNA gene restriction analysis; 16S rRNA gene sequences; Acetic Acid - metabolism; ACETIC ACID BACTERIA; Acetobacter aceti; Acetobacteraceae; Acetobacteraceae - classification; Acetobacteraceae - genetics; Acetobacteraceae - metabolism; Acetobacteraceae - ultrastructure; Ameyamaea chiagmaiensis gen. nov., sp. nov; ARN RIBOSOMAL; ARN RIBOSOMIAL; Bacteria; BACTERIA ACETICA; BACTERIE ACETIQUE; BIODIVERSIDAD; BIODIVERSITE; BIODIVERSITY; Biological and medical sciences; ENZIMA DE RESTRICCION; ENZYME DE RESTRICTION; FILOGENIA; Fundamental and applied biological sciences. Psychology; Gluconacetobacter; Gluconacetobacter liquefaciens; Microscopy, Electron, Transmission; MORFOLOGIA; MORPHOLOGIE; MORPHOLOGY; NUCLEOTIDE SEQUENCE; PCR; PHYLOGENIE; PHYLOGENY; RESTRICTION ENZYMES; RIBOSOMAL RNA; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; TAILANDIA; THAILAND; THAILANDE; Acetobacteraceae; 16S rRNA gene sequences; 16S rRNA gene restriction analysis; Gene; Nucleotide sequence; Ribosomal RNA; Ameyamaea chiagmaiensis gen. nov. sp. nov; 16S-RNA; Bacteria; Acetic acid bacteria
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.90070

Two isolates, AC04 T and AC05, were isolated from the flowers of red ginger collected in Chiang Mai, Thailand. In phylogenetic trees based on 16S rRNA gene sequences, the two isolates were included within a lineage comprised of the genera Acidomonas, Gluconacetobacter, Asaia, Kozakia, Swaminathania, Neoasaia, Granulibacter, and Tanticharoenia, and they formed an independent cluster along with the type strain of Tanticharoenia sakaeratensis. The calculated pair-wise sequence similarities of isolate AC04 T were 97.8-92.5% to the type strains of the type species of the 11 genera of acetic acid bacteria. The DNA base composition was 66.0-66.1 mol % G+C with a range of 0.1 mol %. A single-stranded, labeled DNA from isolate AC04 T presented levels of DNA-DNA hybridization of 100, 85, 4, and 3% respectively to DNAs from isolates AC04 T and AC05 and the type strains of Tanticharoenia sakaeratensis and Gluconacetobacter liquefaciens. The two isolates were unique morphologically in polar flagellation and physiologically in intense acetate oxidation to carbon dioxide and water and weak lactate oxidation. The intensity in acetate oxidation almost equaled that of the type strain of Acetobacter aceti. The two isolates had Q-10. Isolate AC04 T was discriminated from the type strains of the type species of the 11 genera by 16S rRNA gene restriction analysis using restriction endonucleases TaqI and Hin6I. The unique phylogenetic, genetic, morphological, physiological, and biochemical characteristics obtained indicate that the two isolates can be classified into a separate genus, and Ameyamaea chiangmaiensis gen. nov., sp. nov. is proposed. The type strain is isolate AC04 T (=BCC 15744 T , =NBRC 103196 T ), which has a DNA G+C content of 66.0 mol %.