Electric field-driven microfluidics for rapid CRISPR-based diagnostics and its application to detection of SARS-CoV-2

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 117; no. 47; pp. 29518 - 29525
• Main Authors: Ramachandran, Ashwin, Huyke, Diego A., Sharma, Eesha, Sahoo, Malaya K., Huang, ChunHong, Banaei, Niaz, Pinsky, Benjamin A., Santiago, Juan G.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 24.11.2020
• Subjects: Assaying; Coronaviruses; COVID-19; COVID-19 Nucleic Acid Testing - methods; CRISPR; CRISPR-Cas Systems; Deoxyribonucleic acid; Diagnostic systems; DNA; DNA probes; Electric fields; Gene amplification; gRNA; Humans; Isotachophoresis; Isotachophoresis - methods; Microfluidics; Microfluidics - methods; Nasal Mucosa - virology; Physical Sciences; Purification; Ribonucleic acid; RNA; SARS-CoV-2 - genetics; SARS-CoV-2 - isolation & purification; Severe acute respiratory syndrome coronavirus 2; Single-stranded DNA; COVID-19; rapid testing; CRISPR diagnostics; microfluidics; isotachophoresis

The rapid spread of COVID-19 across the world has revealed major gaps in our ability to respond to new virulent pathogens. Rapid, accurate, and easily configurable molecular diagnostic tests are imperative to prevent global spread of new diseases. CRISPR-based diagnostic approaches are proving to be...