Electric field-driven microfluidics for rapid CRISPR-based diagnostics and its application to detection of SARS-CoV-2 - Ramachandran, Ashwin; Huyke, Diego A.; Sharma, Eesha; Sahoo, Malaya K.; Huang, ChunHong; Banaei, Niaz; Pinsky, Benjamin A.; Santiago, Juan G. (Proceedings of the National Academy of Sciences - PNAS, 2020)
Keywords: Assaying; Coronaviruses; COVID-19; COVID-19 Nucleic Acid Testing - methods; CRISPR; CRISPR-Cas Systems; Deoxyribonucleic acid; Diagnostic systems; DNA; DNA probes; Electric fields; Gene amplification; gRNA; Humans; Isotachophoresis; Isotachophoresis - methods; Microfluidics; Microfluidics - methods; Nasal Mucosa - virology; Physical Sciences; Purification; Ribonucleic acid; RNA; SARS-CoV-2 - genetics; SARS-CoV-2 - isolation & purification; Severe acute respiratory syndrome coronavirus 2; Single-stranded DNA; COVID-19; rapid testing; CRISPR diagnostics; microfluidics; isotachophoresis