The in Vivo TRPV6 Protein Starts at a Non-AUG Triplet, Decoded as Methionine, Upstream of Canonical Initiation at AUG

• Published in: The Journal of biological chemistry Vol. 288; no. 23; pp. 16629 - 16644
• Main Authors: Fecher-Trost, Claudia, Wissenbach, Ulrich, Beck, Andreas, Schalkowsky, Pascal, Stoerger, Christof, Doerr, Janka, Dembek, Anna, Simon-Thomas, Martin, Weber, Armin, Wollenberg, Peter, Ruppert, Thomas, Middendorff, Ralf, Maurer, Hans H., Flockerzi, Veit
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.06.2013; American Society for Biochemistry and Molecular Biology
• Subjects: Antibodies; Calcium Channels; Calcium Channels - biosynthesis; Calcium Channels - genetics; Cell Membrane - genetics; Cell Membrane - metabolism; Codon, Initiator - genetics; Codon, Initiator - metabolism; HEK293 Cells; Humans; Mass Spectrometry (MS); Methionine; Protein Biosynthesis - physiology; Protein Transport - physiology; Signal Transduction; Translation; Translation Initiation at Non-AUG; TRP Channels; TRPV Cation Channels - biosynthesis; TRPV Cation Channels - genetics; TRPV6; Translation; Calcium Channels; TRPV6; TRP Channels; Antibodies; Mass Spectrometry (MS); Translation Initiation at Non-AUG

TRPV6 channels function as epithelial Ca2+ entry pathways in the epididymis, prostate, and placenta. However, the identity of the endogenous TRPV6 protein relies on predicted gene coding regions and is only known to a certain level of approximation. We show that in vivo the TRPV6 protein has an exte...