Phosphoproteins in extracellular vesicles as candidate markers for breast cancer

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 114; no. 12; pp. 3175 - 3180
• Main Authors: Chen, I-Hsuan, Xue, Liang, Hsu, Chuan-Chih, Paez, Juan Sebastian Paez, Pan, Li, Andaluz, Hillary, Wendt, Michael K., Iliuk, Anton B., Zhu, Jian-Kang, Tao, W. Andy
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 21.03.2017
• Subjects: Biological Sciences; Biomarkers; Breast cancer; Medical diagnosis; Phosphorylation; Plasma; Proteins; Proteomics; phosphoproteins; mass spectrometry; extracellular vesicles; proteomics; biomarker

The state of protein phosphorylation can be a key determinant of cellular physiology such as early-stage cancer, but the development of phosphoproteins in biofluids for disease diagnosis remains elusive. Here we demonstrate a strategy to isolate and identify phosphoproteins in extracellular vesicles...