Development of an entirely plasmid-based reverse genetics system for 12-segmented double-stranded RNA viruses

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 118; no. 42; pp. 1 - 8
• Main Authors: Nouda, Ryotaro, Minami, Shohei, Kanai, Yuta, Kawagishi, Takahiro, Nurdin, Jeffery A., Yamasaki, Moeko, Kuwata, Ryusei, Shimoda, Hiroshi, Maeda, Ken, Kobayashi, Takeshi
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 19.10.2021
• Subjects: Animals; Biological Sciences; Cricetinae; DNA-directed RNA polymerase; Double-stranded RNA; Genetics; Genome, Viral; Genomes; Glycosylation; Mutation; Plasmids; Proteins; Reassortant Viruses - genetics; Reoviridae; Reoviridae - genetics; Ribonucleic acid; RNA; RNA polymerase; RNA viruses; Rotavirus; Segments; Therapeutic applications; Transfection; Viruses; coltiviruses; Reoviridae viruses; reverse genetics system

The family Reoviridae is a nonenveloped virus group with a double-stranded (ds) RNA genome comprising 9 to 12 segments. In the family Reoviridae, the genera Cardoreovirus, Phytoreovirus, Seadornavirus, Mycoreovirus, and Coltivirus contain virus species having 12-segmented dsRNA genomes. Reverse gene...