Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation

• Published in: The Journal of biological chemistry Vol. 291; no. 3; pp. 1103 - 1114
• Main Authors: Pilecki, Bartosz, Holm, Anne T., Schlosser, Anders, Moeller, Jesper B., Wohl, Alexander P., Zuk, Alexandra V., Heumüller, Stefanie E., Wallis, Russell, Moestrup, Soren K., Sengle, Gerhard, Holmskov, Uffe, Sorensen, Grith L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.01.2016; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Substitution; analytical ultracentrifugation; Animals; calcium-binding protein; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; Desmosine - metabolism; Elastic Tissue - metabolism; elastin; Extracellular Matrix Proteins - chemistry; Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - metabolism; fibrillin; Fibrillin-1; Fibrillins; Glycobiology and Extracellular Matrices; Glycoproteins - chemistry; Glycoproteins - genetics; Glycoproteins - metabolism; Humans; Ligands; Male; Mice, Inbred C57BL; Mice, Mutant Strains; microfibrillar-associated protein 4; Microfibrils - metabolism; Microfilament Proteins - chemistry; Microfilament Proteins - genetics; Microfilament Proteins - metabolism; Models, Molecular; Mutation; Peptide Fragments - chemistry; Peptide Fragments - genetics; Peptide Fragments - metabolism; Protein Interaction Domains and Motifs; Protein Isoforms - chemistry; Protein Isoforms - genetics; Protein Isoforms - metabolism; Protein Multimerization; Protein Transport; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; site-directed mutagenesis; surface plasmon resonance (SPR); Tropoelastin - chemistry; Tropoelastin - genetics; Tropoelastin - metabolism; site-directed mutagenesis; analytical ultracentrifugation; calcium-binding protein; microfibrillar-associated protein 4; surface plasmon resonance (SPR); elastin; fibrillin

MFAP4 (microfibrillar-associated protein 4) is an extracellular glycoprotein found in elastic fibers without a clearly defined role in elastic fiber assembly. In the present study, we characterized molecular interactions between MFAP4 and elastic fiber components. We established that MFAP4 primarily...