The E3 Ubiquitin Ligases, HUWE1 and NEDD4-1, Are Involved in the Post-translational Regulation of the ABCG1 and ABCG4 Lipid Transporters

• Published in: The Journal of biological chemistry Vol. 290; no. 40; pp. 24604 - 24613
• Main Authors: Aleidi, Shereen M., Howe, Vicky, Sharpe, Laura J., Yang, Alryel, Rao, Geetha, Brown, Andrew J., Gelissen, Ingrid C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 02.10.2015; American Society for Biochemistry and Molecular Biology
• Subjects: ABC transporter; Animals; ATP Binding Cassette Transporter, Subfamily G; ATP Binding Cassette Transporter, Subfamily G, Member 1; ATP-Binding Cassette Transporters - metabolism; Biological Transport; Cell Line; CHO Cells; Cholesterol - chemistry; cholesterol regulation; Chromatography, Liquid; Cricetulus; E3 ubiquitin ligase; Endosomal Sorting Complexes Required for Transport - metabolism; Gene Expression Regulation; Gene Silencing; Humans; Lipids; Lipids - chemistry; Macrophages - metabolism; Mass Spectrometry; membrane lipid; Nedd4 Ubiquitin Protein Ligases; post-transcriptional regulation; Protein Binding; Protein Multimerization; Protein Structure, Tertiary; Protein Transport; RNA, Small Interfering - metabolism; Tumor Suppressor Proteins; Ubiquitin-Protein Ligases - metabolism; E3 ubiquitin ligase; cholesterol regulation; ABC transporter; membrane lipid; post-transcriptional regulation

The ATP-binding cassette transporter ABCG1 has an essential role in cellular cholesterol homeostasis, and dysregulation has been associated with a number of high burden diseases. Previous studies reported that ABCG1 is ubiquitinated and degraded via the ubiquitin proteasome system. However, so far t...