7-Hydroperoxycholesterol and its products in oxidized low density lipoprotein and human atherosclerotic plaque

• Published in: Journal of lipid research Vol. 38; no. 9; pp. 1730 - 1745
• Main Authors: Brown, A J, Leong, S L, Dean, R T, Jessup, W
• Format: Journal Article
• Language: English
• Published: United States Elsevier 01.09.1997
• Subjects: Animals; Arteriosclerosis - metabolism; Cholesterol - analogs & derivatives; Cholesterol - analysis; Cholesterol - blood; Cholesterol - metabolism; Chromatography, High Pressure Liquid - methods; Copper; Humans; In Vitro Techniques; Lipid Peroxides - analysis; Lipid Peroxides - blood; Lipoproteins, LDL - blood; Lipoproteins, LDL - chemistry; Macrophages, Peritoneal - metabolism; Mice; Oxidation-Reduction

7-Hydroperoxycholesterols (7OOHs) are intermediates in cholesterol oxidation and potential cytotoxins. A normal-phase HPLC method with UV (205 nm) detection was developed that could resolve 7 alpha OOH, 7 beta OOH, 7-ketocholesterol (7K), and the epimeric 7-hydroxycholesterols (7OHs). 7OOH formation...