Paf1C regulates RNA polymerase II progression by modulating elongation rate

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 116; no. 29; pp. 14583 - 14592
• Main Authors: Hou, Liming, Wang, Yating, Liu, Yu, Zhang, Nan, Shamovsky, Ilya, Nudler, Evgeny, Tian, Bin, Dynlacht, Brian David
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 16.07.2019
• Series: PNAS Plus
• Subjects: Ablation; Accumulation; Animals; Biological Sciences; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cell Line; Compression; CRISPR-Cas Systems - genetics; DNA-directed RNA polymerase; Elongation; Gene Knockout Techniques; Histone H2B; Histones - metabolism; Mammalian cells; Mice; Myoblasts; Occupancy; PNAS Plus; Promoter Regions, Genetic - genetics; Ribonucleic acid; RNA; RNA polymerase; RNA polymerase II; RNA Polymerase II - metabolism; RNA, Small Interfering - metabolism; Transcription; Transcription Elongation, Genetic; Transcription Termination, Genetic; Ubiquitin; Ubiquitination - genetics; Paf1 complex; nucleosome occupancy; transcription elongation rate; RNA polymerase II

Elongation factor Paf1C regulates several stages of the RNA polymerase II (Pol II) transcription cycle, although it is unclear how it modulates Pol II distribution and progression in mammalian cells. We found that conditional ablation of Paf1 resulted in the accumulation of unphosphorylated and Ser5...