In vivo imaging of the pathophysiological changes and neutrophil dynamics in influenza virus-infected mouse lungs

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 115; no. 28; pp. E6622 - E6629
• Main Authors: Ueki, Hiroshi, Wang, I-Hsuan, Fukuyama, Satoshi, Katsura, Hiroaki, da Silva Lopes, Tiago Jose, Neumann, Gabriele, Kawaoka, Yoshihiro
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 10.07.2018
• Series: PNAS Plus
• Subjects: Animals; Biological Sciences; Fluorescence; Histopathology; Imaging; Influenza; Influenza A Virus, H5N1 Subtype - genetics; Influenza A Virus, H5N1 Subtype - metabolism; Information systems; Leukocytes (neutrophilic); Lung - metabolism; Lung - pathology; Lung - virology; Lung diseases; Lungs; Mice; Microscopy, Fluorescence, Multiphoton; Neutrophils; Orthomyxoviridae; Orthomyxoviridae Infections - genetics; Orthomyxoviridae Infections - metabolism; Orthomyxoviridae Infections - pathology; Pathogenicity; Pathogens; Perfusion; Permeability; Physiology; PNAS Plus; Viruses; lung; influenza virus; in vivo imaging; neutrophil dynamics; two-photon excitation microscopy

The pathophysiological changes that occur in lungs infected with influenza viruses are poorly understood. Here we established an in vivo imaging system that combines two-photon excitation microscopy and fluorescent influenza viruses of different pathogenicity. This approach allowed us to monitor and...