Error-prone Translesion Synthesis Past DNA-Peptide Cross-links Conjugated to the Major Groove of DNA via C5 of Thymidine

• Published in: The Journal of biological chemistry Vol. 290; no. 2; pp. 775 - 787
• Main Authors: Wickramaratne, Susith, Boldry, Emily J., Buehler, Charles, Wang, Yen-Chih, Distefano, Mark D., Tretyakova, Natalia Y.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09.01.2015; American Society for Biochemistry and Molecular Biology
• Subjects: Cancer; Click Chemistry; DNA Adducts - chemistry; DNA Adducts - metabolism; DNA and Chromosomes; DNA Damage; DNA Damage - genetics; DNA Polymerase; DNA Repair - genetics; DNA Replication; DNA-Directed DNA Polymerase - metabolism; DNA-Protein Conjugates; Humans; Kinetics; Mass Spectrometry (MS); Mutations; Neoplasms - metabolism; Neoplasms - pathology; Peptides - chemistry; Peptides - genetics; Reactive Oxygen Species - metabolism; Steady-state Kinetics; Tandem Mass Spectrometry; Thymidine - chemistry; Thymidine - genetics; Translesion Synthesis; Steady-state Kinetics; Mutations; DNA Polymerase; Translesion Synthesis; Click Chemistry; Mass Spectrometry (MS); DNA-Protein Conjugates; DNA Damage; Cancer

DNA-protein cross-links (DPCs) are exceptionally bulky, structurally diverse DNA adducts formed in cells upon exposure to endogenous and exogenous bis-electrophiles, reactive oxygen species, and ionizing radiation. If not repaired, DPCs can induce toxicity and mutations. It has been proposed that th...