Molecular Detection of Genetic Material of Toxoplasma gondii in Goat Blood Samples from Northern Thailand
Toxoplasmosis is a significant parasitic zoonosis caused by Toxoplasma gondii. Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. The present study ai...
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| Published in | Veterinary sciences Vol. 12; no. 6; p. 555 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
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05.06.2025
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| Abstract | Toxoplasmosis is a significant parasitic zoonosis caused by Toxoplasma gondii. Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. The present study aimed to assess the prevalence of T. gondii infection in goats in northern Thailand, with an emphasis on its potential zoonotic transmission to humans. Polymerase chain reaction (PCR) targeting the T. gondii dihydrofolate reductase-thymidylate synthase (Tgdhfr-ts) gene was employed for molecular detection. This represents the first report of T. gondii molecular detection in blood samples from small ruminants in Thailand by PCR. A total of 176 meat goat blood samples were analyzed, yielding an 8.52% (15/176) positivity rate for T. gondii DNA. The selected DNA sequences from the positive T. gondii DNA displayed a high degree of nucleotide sequence homology with the reference Tgdhfr-ts sequence. Phylogenetic analysis revealed a single clade alongside other T. gondii strains, showing no differentiation based on genotype. This study contributes to the understanding of T. gondii epidemiology and provides a foundation for future strategies to control and manage T. gondii transmission in livestock populations. |
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| AbstractList | Toxoplasmosis is a significant parasitic zoonosis caused by Toxoplasma gondii. Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. The present study aimed to assess the prevalence of T. gondii infection in goats in northern Thailand, with an emphasis on its potential zoonotic transmission to humans. Polymerase chain reaction (PCR) targeting the T. gondii dihydrofolate reductase-thymidylate synthase (Tgdhfr-ts) gene was employed for molecular detection. This represents the first report of T. gondii molecular detection in blood samples from small ruminants in Thailand by PCR. A total of 176 meat goat blood samples were analyzed, yielding an 8.52% (15/176) positivity rate for T. gondii DNA. The selected DNA sequences from the positive T. gondii DNA displayed a high degree of nucleotide sequence homology with the reference Tgdhfr-ts sequence. Phylogenetic analysis revealed a single clade alongside other T. gondii strains, showing no differentiation based on genotype. This study contributes to the understanding of T. gondii epidemiology and provides a foundation for future strategies to control and manage T. gondii transmission in livestock populations. Toxoplasmosis is a significant parasitic zoonosis caused by . Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. The present study aimed to assess the prevalence of infection in goats in northern Thailand, with an emphasis on its potential zoonotic transmission to humans. Polymerase chain reaction (PCR) targeting the dihydrofolate reductase-thymidylate synthase ( ) gene was employed for molecular detection. This represents the first report of molecular detection in blood samples from small ruminants in Thailand by PCR. A total of 176 meat goat blood samples were analyzed, yielding an 8.52% (15/176) positivity rate for DNA. The selected DNA sequences from the positive DNA displayed a high degree of nucleotide sequence homology with the reference sequence. Phylogenetic analysis revealed a single clade alongside other strains, showing no differentiation based on genotype. This study contributes to the understanding of epidemiology and provides a foundation for future strategies to control and manage transmission in livestock populations. Toxoplasmosis, caused by the parasite Toxoplasma gondii, can be transmitted from animals to humans. Sheep and goats are particularly susceptible, resulting in economic losses for farmers. This study examined the presence of the parasite in goats in northern Thailand using an alternative DNA target in the PCR method. Of 176 goats tested, 8.52% were positive. These findings provide valuable insights into the disease’s transmission and support future control efforts. Toxoplasmosis is a significant parasitic zoonosis caused by Toxoplasma gondii. Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. The present study aimed to assess the prevalence of T. gondii infection in goats in northern Thailand, with an emphasis on its potential zoonotic transmission to humans. Polymerase chain reaction (PCR) targeting the T. gondii dihydrofolate reductase-thymidylate synthase (Tgdhfr-ts) gene was employed for molecular detection. This represents the first report of T. gondii molecular detection in blood samples from small ruminants in Thailand by PCR. A total of 176 meat goat blood samples were analyzed, yielding an 8.52% (15/176) positivity rate for T. gondii DNA. The selected DNA sequences from the positive T. gondii DNA displayed a high degree of nucleotide sequence homology with the reference Tgdhfr-ts sequence. Phylogenetic analysis revealed a single clade alongside other T. gondii strains, showing no differentiation based on genotype. This study contributes to the understanding of T. gondii epidemiology and provides a foundation for future strategies to control and manage T. gondii transmission in livestock populations.Toxoplasmosis is a significant parasitic zoonosis caused by Toxoplasma gondii. Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. The present study aimed to assess the prevalence of T. gondii infection in goats in northern Thailand, with an emphasis on its potential zoonotic transmission to humans. Polymerase chain reaction (PCR) targeting the T. gondii dihydrofolate reductase-thymidylate synthase (Tgdhfr-ts) gene was employed for molecular detection. This represents the first report of T. gondii molecular detection in blood samples from small ruminants in Thailand by PCR. A total of 176 meat goat blood samples were analyzed, yielding an 8.52% (15/176) positivity rate for T. gondii DNA. The selected DNA sequences from the positive T. gondii DNA displayed a high degree of nucleotide sequence homology with the reference Tgdhfr-ts sequence. Phylogenetic analysis revealed a single clade alongside other T. gondii strains, showing no differentiation based on genotype. This study contributes to the understanding of T. gondii epidemiology and provides a foundation for future strategies to control and manage T. gondii transmission in livestock populations. Toxoplasmosis, caused by the parasite Toxoplasma gondii , can be transmitted from animals to humans. Sheep and goats are particularly susceptible, resulting in economic losses for farmers. This study examined the presence of the parasite in goats in northern Thailand using an alternative DNA target in the PCR method. Of 176 goats tested, 8.52% were positive. These findings provide valuable insights into the disease’s transmission and support future control efforts. Toxoplasmosis is a significant parasitic zoonosis caused by Toxoplasma gondii . Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. The present study aimed to assess the prevalence of T. gondii infection in goats in northern Thailand, with an emphasis on its potential zoonotic transmission to humans. Polymerase chain reaction (PCR) targeting the T. gondii dihydrofolate reductase-thymidylate synthase ( Tgdhfr-ts ) gene was employed for molecular detection. This represents the first report of T. gondii molecular detection in blood samples from small ruminants in Thailand by PCR. A total of 176 meat goat blood samples were analyzed, yielding an 8.52% (15/176) positivity rate for T. gondii DNA. The selected DNA sequences from the positive T. gondii DNA displayed a high degree of nucleotide sequence homology with the reference Tgdhfr-ts sequence. Phylogenetic analysis revealed a single clade alongside other T. gondii strains, showing no differentiation based on genotype. This study contributes to the understanding of T. gondii epidemiology and provides a foundation for future strategies to control and manage T. gondii transmission in livestock populations. |
| Audience | Academic |
| Author | Apinda, Nisachon Maneekesorn, Supawadee Koonyosying, Pongpisid Sangkakam, Kanokwan Muenthaisong, Anucha Rittipornlertrak, Amarin Nontasaya, Kanpitcha Nambooppha, Boondarika Sthitmatee, Nattawooti |
| AuthorAffiliation | 3 Division of Hematology and Oncology, Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand 1 Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand; anucha.m@cmu.ac.th (A.M.); kanokwansangkakam@gmail.com (K.S.); n.nontasaya@gmail.com (K.N.); amarin.r@cmu.ac.th (A.R.); boondarika.n@cmu.ac.th (B.N.); nisachon.a@cmu.ac.th (N.A.); supawadee.man@cmu.ac.th (S.M.); nattawooti.s@cmu.ac.th (N.S.) 2 Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand |
| AuthorAffiliation_xml | – name: 1 Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand; anucha.m@cmu.ac.th (A.M.); kanokwansangkakam@gmail.com (K.S.); n.nontasaya@gmail.com (K.N.); amarin.r@cmu.ac.th (A.R.); boondarika.n@cmu.ac.th (B.N.); nisachon.a@cmu.ac.th (N.A.); supawadee.man@cmu.ac.th (S.M.); nattawooti.s@cmu.ac.th (N.S.) – name: 3 Division of Hematology and Oncology, Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand – name: 2 Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand |
| Author_xml | – sequence: 1 givenname: Pongpisid orcidid: 0000-0002-5311-2220 surname: Koonyosying fullname: Koonyosying, Pongpisid – sequence: 2 givenname: Anucha orcidid: 0000-0002-0126-0758 surname: Muenthaisong fullname: Muenthaisong, Anucha – sequence: 3 givenname: Kanokwan orcidid: 0009-0004-8747-2912 surname: Sangkakam fullname: Sangkakam, Kanokwan – sequence: 4 givenname: Kanpitcha surname: Nontasaya fullname: Nontasaya, Kanpitcha – sequence: 5 givenname: Amarin surname: Rittipornlertrak fullname: Rittipornlertrak, Amarin – sequence: 6 givenname: Boondarika orcidid: 0000-0002-9951-9915 surname: Nambooppha fullname: Nambooppha, Boondarika – sequence: 7 givenname: Nisachon surname: Apinda fullname: Apinda, Nisachon – sequence: 8 givenname: Supawadee surname: Maneekesorn fullname: Maneekesorn, Supawadee – sequence: 9 givenname: Nattawooti orcidid: 0000-0002-2329-8802 surname: Sthitmatee fullname: Sthitmatee, Nattawooti |
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| Cites_doi | 10.1111/tbed.14753 10.4103/0971-5916.92633 10.14202/vetworld.2021.2386-2391 10.1099/00222615-32-1-25 10.1016/j.cimid.2022.101928 10.1021/acschembio.1c00627 10.1051/parasite/2024038 10.1111/jfs.12425 10.1007/s004360050248 10.1128/JCM.00898-10 10.1016/S0020-7519(99)00170-8 10.1016/j.vetpar.2004.09.005 10.1093/bioinformatics/btg109 10.1128/jcm.27.8.1787-1792.1989 10.14202/vetworld.2022.1779-1784 10.1051/parasite/2018024 10.3390/ani13172696 10.1021/ml400208v 10.1016/S0304-4017(96)01025-4 10.1016/S0035-9203(02)90078-7 10.1371/journal.pmed.1001920 10.1089/fpd.2008.0223 10.3389/fvets.2021.615310 10.1016/j.cimid.2020.101530 10.1155/2013/905326 10.1016/j.actatropica.2016.03.035 10.1046/j.1365-3156.2000.00632.x 10.1021/acs.jmedchem.8b01754 10.3390/pathogens12040547 10.1016/S0001-706X(01)00084-5 10.1016/j.rvsc.2020.06.014 10.1016/j.vetpar.2004.08.019 10.1016/S0020-7519(00)00124-7 10.1007/s11033-022-07266-8 10.1080/21505594.2021.2012346 10.56808/2985-1130.2928 |
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| Keywords | dihydrofolate reductase thymidylate synthase (dhfr-ts) goat blood samples Toxoplasma gondii molecular detection goat toxoplasmosis polymerase chain reaction (PCR) |
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| Snippet | Toxoplasmosis is a significant parasitic zoonosis caused by Toxoplasma gondii. Among livestock animals, small ruminants, especially sheep and goats, are the... Toxoplasmosis is a significant parasitic zoonosis caused by . Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This... Toxoplasmosis, caused by the parasite Toxoplasma gondii, can be transmitted from animals to humans. Sheep and goats are particularly susceptible, resulting in... Toxoplasmosis, caused by the parasite Toxoplasma gondii , can be transmitted from animals to humans. Sheep and goats are particularly susceptible, resulting in... |
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| SubjectTerms | Abortion Antigens Cattle Dihydrofolate reductase dihydrofolate reductase thymidylate synthase (dhfr-ts) Disease transmission Enzymes Epidemiology Ethylenediaminetetraacetic acid Farms Genes Genetic engineering Genetic testing Genotypes goat blood samples goat toxoplasmosis Goats Homology Infections Livestock Medical research Medicine, Experimental molecular detection Nucleotide sequence Parasites Phylogenetics Polymerase chain reaction polymerase chain reaction (PCR) Protozoa Provinces Public health Ribosomal DNA Serology Software Thymidylate synthase Toxoplasma gondii Toxoplasmosis Veterinary medicine Zoonoses |
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| Title | Molecular Detection of Genetic Material of Toxoplasma gondii in Goat Blood Samples from Northern Thailand |
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