Hdm2 and Nitric Oxide Radicals Contribute to the P53 -Dependent Radioadaptive Response

• Published in: International journal of radiation oncology, biology, physics Vol. 71; no. 2; pp. 550 - 558
• Main Authors: Takahashi, Akihisa, Ph.D, Matsumoto, Hideki, Ph.D, Ohnishi, Takeo, Ph.D
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2008
• Subjects: Cell Line, Tumor; Cell Survival; CHROMOSOMAL ABERRATIONS; Chromosome Aberrations; Cyclic N-Oxides - pharmacology; DICENTRIC CHROMOSOMES; Free Radical Scavengers - pharmacology; Genes, p53 - genetics; Guanidines - pharmacology; Hdm2; Hematology, Oncology and Palliative Medicine; Humans; Imidazoles - pharmacology; LOW DOSE IRRADIATION; LUNGS; NEOPLASMS; NITRIC OXIDE; Nitric Oxide - pharmacology; Nitric Oxide Synthase - pharmacology; Nitric Oxide Synthase Type II - antagonists & inhibitors; Nitric Oxide Synthase Type II - metabolism; p53; Piperazines - pharmacology; Proto-Oncogene Proteins c-mdm2 - antagonists & inhibitors; Proto-Oncogene Proteins c-mdm2 - metabolism; Proto-Oncogene Proteins c-mdm2 - radiation effects; Radiation Tolerance - genetics; Radiation Tolerance - physiology; Radioadaptive response; RADIOBIOLOGY; Radiology; RADIOLOGY AND NUCLEAR MEDICINE; RADIOSENSITIVITY; Time Factors; Tumor Suppressor Protein p53 - antagonists & inhibitors; Tumor Suppressor Protein p53 - metabolism; NO; Radiobiology; Hdm2; Radioadaptive response; p53

Purpose The aim of this work was to characterize the radioadaptive response at the molecular level. Methods and Materials We used wild-type (wt) p53 and mutated (m) p53- containing cells derived from the human lung cancer H1299 cell line, which is p53 -null. Cellular radiation sensitivities were det...