Mapping the Protein Interaction Network of the Human COP9 Signalosome Complex Using a Label-free QTAX Strategy

• Published in: Molecular & cellular proteomics Vol. 11; no. 5; pp. 138 - 147
• Main Authors: Fang, Lei, Kaake, Robyn M., Patel, Vishal R., Yang, Yingying, Baldi, Pierre, Huang, Lan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2012; The American Society for Biochemistry and Molecular Biology
• Subjects: Autoantigens - chemistry; Autoantigens - isolation & purification; Autoantigens - metabolism; Chromatography, Affinity; COP9 Signalosome Complex; Cross-Linking Reagents - chemistry; Formaldehyde - chemistry; HeLa Cells; Humans; Immunoprecipitation; Molecular Sequence Annotation; Multiprotein Complexes - isolation & purification; Multiprotein Complexes - metabolism; Peptide Fragments - chemistry; Peptide Hydrolases - isolation & purification; Peptide Hydrolases - metabolism; Peptide Mapping; Proteasome Endopeptidase Complex - chemistry; Proteasome Endopeptidase Complex - isolation & purification; Proteasome Endopeptidase Complex - metabolism; Protein Binding; Protein Interaction Mapping; Protein Interaction Maps; Proteolysis

The COP9 signalosome (CSN) is a multi-subunit protein complex that performs critical roles in controlling diverse cellular and developmental processes. Aberrant regulation of the CSN complex has been shown to lead to tumorigenesis. Despite its biological significance, our current knowledge of the fu...