Three-dimensional deconvolution processing for STEM cryotomography

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 117; no. 44; pp. 27374 - 27380
• Main Authors: Waugh, Barnali, Wolf, Sharon G., Fass, Deborah, Branlund, Eric, Kam, Zvi, Sedat, John W., Elbaum, Michael
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 03.11.2020
• Subjects: Algorithms; Biological Sciences; Cell Line; Chromatin; Computer applications; Crosstalk; Deconvolution; Electron Microscope Tomography - methods; Fluorescence; Fluorescence microscopy; Frozen Sections; Gold Colloid; Haze; Humans; Image Enhancement - methods; Imaging, Three-Dimensional; Microscopy, Electron, Scanning Transmission - methods; Noise reduction; Planes; Point spread functions; Tomography; chromatin; tomography; cryoelectron microscopy

The complex environment of biological cells and tissues has motivated development of three-dimensional (3D) imaging in both light and electron microscopies. To this end, one of the primary tools in fluorescence microscopy is that of computational deconvolution. Wide-field fluorescence images are oft...