A method comparison study of the high throughput automated HISCL® SARS‐CoV‐2 antigen assay using nasopharyngeal swab samples from symptomatic and asymptomatic subjects against conventional RT‐PCR

• Published in: Journal of medical virology Vol. 94; no. 7; pp. 3070 - 3080
• Main Authors: Linssen, Joachim, Schapendonk, Claire, Münster, Marion, Daemen, Paul, Rahamat‐Langendoen, Janette, Wertheim, Heiman
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.07.2022; John Wiley and Sons Inc
• Subjects: Antigens; Assaying; Automation; Confidence intervals; Coronaviruses; Health care; HISCL automated antigen assay; Medical personnel; method comparison; Performance evaluation; Polymerase chain reaction; Public health; rapid testing; RT‐PCR; SARS‐CoV‐2; Sensitivity; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Transport media; variants of concern; Viral diseases; Virology; SARS-CoV-2; rapid testing; method comparison; variants of concern; HISCL automated antigen assay; RT-PCR

Our study aim was to evaluate the performance of the automated Sysmex HISCL® severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) antigen assay against reverse‐transcription polymerase chain reaction (RT‐PCR). We tested 277 remnant frozen nasopharyngeal swab samples, stored in universal tran...