Elevation in Cytosolic Free Calcium Concentration Early in Myocardial Ischemia in Perfused Rat Heart

• Published in: Circulation research Vol. 60; no. 5; pp. 700 - 707
• Main Authors: Steenbergen, Charles, Murphy, Elizabeth, Levy, Louis, London, Robert E
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD American Heart Association, Inc 01.05.1987; Lippincott
• Subjects: Animals; Biological and medical sciences; Calcium - metabolism; Cardiology. Vascular system; Coronary Disease - metabolism; Coronary heart disease; Cytosol - metabolism; Egtazic Acid; Heart; In Vitro Techniques; Magnetic Resonance Spectroscopy; Male; Medical sciences; Myocardium - metabolism; Perfusion; Rats; Rats, Inbred Strains; Vertebrata; Experimental disease; Mammalia; Calcium; Ischemia; Rat; Rodentia; Myocardium; Cardiovascular disease; Coronary heart disease; Cytosol; In vitro

Changes in cytosolic free calcium concentration during myocardial ischemia were measured by F NMR in 5FBAPTA-loaded perfused rat hearts. The hearts were perfused with Krebs-Henseleit buffer containing 5 μM of the acetoxymethyl ester of 5FBAPTA, which was hydrolyzed by cytosolic esterases to achieve...