Podocyte Activation of NLRP3 Inflammasomes Contributes to the Development of Proteinuria in Lupus Nephritis

• Published in: Arthritis & rheumatology (Hoboken, N.J.) Vol. 69; no. 8; pp. 1636 - 1646
• Main Authors: Fu, Rong, Guo, Chaohuan, Wang, Shuang, Huang, Yuefang, Jin, Ou, Hu, Haoqiang, Chen, Jingxian, Xu, Bihua, Zhou, Mianjing, Zhao, Jijun, Sung, Sun‐sang J., Wang, Hongyang, Gaskin, Felicia, Yang, Niansheng, Fu, Shu Man
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.08.2017
• Subjects: Activation; Activation analysis; Animals; Autoimmune diseases; Biopsy; Blotting, Western; Caspase; Caspase 1 - drug effects; Caspase 1 - immunology; Caspase 1 - metabolism; Caspase-1; Cell Line; Cytometry; Feet; Flow Cytometry; Fluorescence; Heterocyclic Compounds, 4 or More Rings - pharmacology; Humans; In vitro methods and tests; Inflammasomes; Inhibitors; Interleukin-1beta - drug effects; Interleukin-1beta - immunology; Interleukin-1beta - metabolism; Kidney - drug effects; Kidney - immunology; Kidney - metabolism; Kidney - ultrastructure; Kidney Glomerulus - drug effects; Kidney Glomerulus - immunology; Kidney Glomerulus - metabolism; Kidney Glomerulus - ultrastructure; Kidneys; Lesions; Lupus; Lupus nephritis; Lupus Nephritis - immunology; Lupus Nephritis - metabolism; Lupus Nephritis - pathology; Mice; Microscopy, Confocal; Microscopy, Electron, Transmission; Nephritis; NLR Family, Pyrin Domain-Containing 3 Protein - antagonists & inhibitors; NLR Family, Pyrin Domain-Containing 3 Protein - immunology; Pathogenesis; Patients; Podocytes - drug effects; Podocytes - immunology; Podocytes - ultrastructure; Proteinuria; Proteinuria - immunology; Proteinuria - metabolism; Proteinuria - pathology; Reactive oxygen species; Reactive Oxygen Species - metabolism; Rodents; Sulfones - pharmacology; Tissues; Ultrastructure

Objective Development of proteinuria in lupus nephritis (LN) is associated with podocyte dysfunction. The NLRP3 inflammasome has been implicated in the pathogenesis of LN. The purpose of this study was to investigate whether NLRP3 inflammasome activation is involved in the development of podocyte in...