Hepatocellular Carcinoma Detection by Plasma Methylated DNA: Discovery, Phase I Pilot, and Phase II Clinical Validation

• Published in: Hepatology (Baltimore, Md.) Vol. 69; no. 3; pp. 1180 - 1192
• Main Authors: Kisiel, John B., Dukek, Brian A., V.S.R. Kanipakam, Reddappa, Ghoz, Hassan M., Yab, Tracy C., Berger, Calise K., Taylor, William R., Foote, Patrick H., Giama, Nasra H., Onyirioha, Kristeen, Abdallah, Mohamed A., Burger, Kelli N., Slettedahl, Seth W., Mahoney, Douglas W., Smyrk, Thomas C., Lewis, Jason T., Giakoumopoulos, Maria, Allawi, Hatim T., Lidgard, Graham P., Roberts, Lewis R., Ahlquist, David A.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.03.2019
• Subjects: Bisulfite; Carcinoma, Hepatocellular; Cirrhosis; Deoxyribonucleic acid; DNA; DNA Methylation; DNA sequencing; DNA, Neoplasm - blood; DNA, Neoplasm - metabolism; Early Detection of Cancer - methods; Endothelins; Female; Hepatocellular carcinoma; Hepatology; Homeobox; HOXA1 protein; Humans; Liver cancer; Liver cirrhosis; Liver Neoplasms - blood; Male; Middle Aged; Phosphofructokinase; Pilot Projects; Plasma; Regression analysis; Single-Blind Method; Spiracles

Early detection improves hepatocellular carcinoma (HCC) outcomes, but better noninvasive surveillance tools are needed. We aimed to identify and validate methylated DNA markers (MDMs) for HCC detection. Reduced representation bisulfite sequencing was performed on DNA extracted from 18 HCC and 35 con...