Modified SHI medium supports growth of a disease‐state subgingival polymicrobial community in vitro

• Published in: Molecular oral microbiology Vol. 36; no. 1; pp. 37 - 49
• Main Authors: Lamont, Eleanor I., Gadkari, Archita, Kerns, Kristopher A., To, Thao T., Daubert, Diane, Kotsakis, Georgios, Bor, Batbileg, He, Xuesong, McLean, Jeffrey S.
• Format: Journal Article
• Language: English
• Published: Denmark Wiley Subscription Services, Inc 01.02.2021; John Wiley and Sons Inc
• Subjects: Biofilms; Dental Plaque; Fetal calf serum; Gingiva; Gram-negative bacteria; Gum disease; Humans; In vitro biofilm model; Inoculum; Mucin; Nutrients; Original; Periodontal Pocket; Periodontitis; RNA, Ribosomal, 16S - genetics; rRNA 16S; SHI‐media; subgingival microbiota; Sucrose; Sugar; Treponema; subgingival microbiota; SHI-media; periodontitis; In vitro biofilm model

Developing a laboratory model of oral polymicrobial communities is essential for in vitro studies of the transition from healthy to diseased oral plaque. SHI medium is an enriched growth medium capable of supporting in vitro biofilms with similar diversity to healthy supragingival inocula; however,...