Single‐Cell RNA Sequencing of Calvarial and Long‐Bone Endocortical Cells

• Published in: Journal of bone and mineral research Vol. 35; no. 10; pp. 1981 - 1991
• Main Authors: Ayturk, Ugur M, Scollan, Joseph P, Goz Ayturk, Didem, Suh, Eun Sung, Vesprey, Alexander, Jacobsen, Christina M, Divieti Pajevic, Paola, Warman, Matthew L
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.10.2020; Wiley Subscription Services, Inc
• Subjects: ANIMAL MODELS; Animals; CELLS OF BONE; Gene expression; Gene Expression Profiling; Kinases; Long bone; Macrophages; Mice; Neonates; OSTEOBLASTS; Osteocytes; Ribonucleic acid; RNA; Sequence Analysis, RNA; Single-Cell Analysis; Skeleton; SOST protein; STATISTICAL METHODS; STROMAL/STEM CELLS; Transcriptome; CELLS OF BONE; STROMAL/STEM CELLS; ANIMAL MODELS; STATISTICAL METHODS; OSTEOBLASTS

ABSTRACT Single‐cell RNA sequencing (scRNA‐Seq) is emerging as a powerful technology to examine transcriptomes of individual cells. We determined whether scRNA‐Seq could be used to detect the effect of environmental and pharmacologic perturbations on osteoblasts. We began with a commonly used in vit...