Diadenosine tetraphosphate induces tight junction disassembly thus increasing corneal epithelial permeability

• Published in: British journal of pharmacology Vol. 172; no. 4; pp. 1045 - 1058
• Main Authors: Loma, P, Guzman‐Aranguez, A, Pérez de Lara, M J, Pintor, J
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.02.2015; BlackWell Publishing Ltd
• Subjects: Animals; Butadienes - pharmacology; Cell Line; Claudins - metabolism; Dinucleoside Phosphates - pharmacology; Epithelial Cells; Epithelium, Corneal - drug effects; Epithelium, Corneal - metabolism; Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors; Humans; Male; MAP Kinase Signaling System - drug effects; Nitriles - pharmacology; Occludin - metabolism; Permeability - drug effects; Rabbits; Receptors, Purinergic P2Y - genetics; Research Papers; RNA, Small Interfering - pharmacology; Tight Junctions - drug effects; Zonula Occludens-1 Protein - metabolism

Background and Purpose Here, we have studied the effects of the dinucleotide P1, P4‐Di (adenosine‐5′) tetraphosphate (Ap4A) on corneal barrier function conferred by the tight junction (TJ) proteins and its possible involvement in ocular drug delivery and therapeutic efficiency. Experimental Approach...