Proteomic profiling of secretome and adherent plasma membranes from distinct mammary epithelial cell subpopulations

• Published in: Proteomics (Weinheim) Vol. 11; no. 20; pp. 4029 - 4039
• Main Authors: Ji, Hong, Goode, Robert J. A., Vaillant, Francois, Mathivanan, Suresh, Kapp, Eugene A., Mathias, Rommel A., Lindeman, Geoffrey J., Visvader, Jane E., Simpson, Richard J.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.10.2011; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: Animals; Cell biology; Cell Line; Cell Membrane - metabolism; Cells, Cultured; Differentiation; ephrin receptors; ephrins; Epithelial cells; Epithelial Cells - cytology; Epithelial Cells - metabolism; Extracellular matrix; Female; Gene Expression Profiling; Integrins; Mammary gland; Mammary Glands, Animal - cytology; Mammary Glands, Animal - metabolism; Mammary stem cell niche; Membranes; Mice; Plasma; Plasma membrane; Plasma membranes; Protein-tyrosine kinase; Proteomics; Rodents; Secretome; Signal Transduction; Stem cells; stromal cells; Tandem Mass Spectrometry; Wnt protein; Wnt10a

The stem cell niche comprises stem cells (SCs), stromal cells, soluble factors, extracellular matrix constituents and vascular networks. The ability to identify signals that regulate SC self‐renewal and differentiation is confounded by the difficulty in isolating pure SC niche components in sufficie...