Cell cycle regulation of human WEE1

• Published in: The EMBO journal Vol. 14; no. 10; pp. 2166 - 2175
• Main Authors: McGowan, C.H., Russell, P.
• Format: Journal Article
• Language: English
• Published: England 15.05.1995
• Subjects: CDC2 Protein Kinase - metabolism; Cell Compartmentation; Cell Cycle - physiology; Cell Cycle Proteins; Cyclin-Dependent Kinases - metabolism; Cyclins - metabolism; DNA Replication; Electrophoresis, Polyacrylamide Gel; G2 Phase - physiology; Gene Expression Regulation; HeLa Cells; Humans; Mitosis - physiology; Molecular Weight; Nuclear Proteins - isolation & purification; Phosphorylation; Protein-Tyrosine Kinases - biosynthesis; Protein-Tyrosine Kinases - chemistry; Protein-Tyrosine Kinases - genetics; Recombinant Proteins - biosynthesis; S Phase - physiology; Subcellular Fractions - physiology; Transfection

WEE1 kinase negatively regulates entry into mitosis by catalyzing the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase. We report here an investigation of human WEE1. Endogenous WEE1 migrates as an approximately 94 kDa protein in SDS‐PAGE, substantially larger than the 49 kDa protein enco...