Low-temperature culture enhances production of flavivirus virus-like particles in mammalian cells

• Published in: Applied microbiology and biotechnology Vol. 108; no. 1; p. 242
• Main Authors: Fan, Yi-Chin, Chen, Jo-Mei, Chen, Yi-Ying, Hsu, Wei-Li, Chang, Gwong-Jen, Chiou, Shyan-Song
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2024; Springer Nature B.V
• Subjects: Amino acids; Animals; Binding; Biomedical and Life Sciences; Biotechnological Products and Process Engineering; Biotechnology; Cell culture; Cell lines; Chlorocebus aethiops; Cold Temperature; COS Cells; Cultivation; Dengue fever; Dengue virus; diagnostic techniques; Encephalitis; Encephalitis Virus, Japanese - genetics; Encephalitis, Japanese; Env protein; Flavivirus; Flavivirus - genetics; genotype; Genotypes; Japanese encephalitis virus; Life Sciences; Low temperature; Mammalian cells; Mammals; Microbial Genetics and Genomics; Microbiology; Monoclonal antibodies; Mutation; Optimization; Plasmids; Protein structure; Proteins; Temperature; Vector-borne diseases; Virus-like particles; Viruses; West Nile virus; Flavivirus; Virus-like particles; Mammalian cells; Cultivation condition

Flavivirus virus-like particles (VLPs) exhibit a striking structural resemblance to viral particles, making them highly adaptable for various applications, including vaccines and diagnostics. Consequently, increasing VLPs production is important and can be achieved by optimizing expression plasmids...