Three-dimensional Printed Mg-Doped β-TCP Bone Tissue Engineering Scaffolds: Effects of Magnesium Ion Concentration on Osteogenesis and Angiogenesis In Vitro
Three-dimensional (3D) printed bone tissue engineering scaffolds have been widely used in research and clinical applications. β-TCP is a biomaterial commonly used in bone tissue engineering to treat bone defects, and its multifunctionality can be achieved by co-doping different metal ions. Magnesium...
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Published in | Tissue engineering and regenerative medicine Vol. 16; no. 4; p. 415 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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Korea (South)
01.08.2019
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Abstract | Three-dimensional (3D) printed bone tissue engineering scaffolds have been widely used in research and clinical applications. β-TCP is a biomaterial commonly used in bone tissue engineering to treat bone defects, and its multifunctionality can be achieved by co-doping different metal ions. Magnesium doping in biomaterials has been shown to alter physicochemical properties of cells and enhance osteogenesis.
A series of Mg-doped TCP scaffolds were manufactured by using cryogenic 3D printing technology and sintering. The characteristics of the porous scaffolds, such as microstructure, chemical composition, mechanical properties, apparent porosity, etc., were examined. To further study the role of magnesium ions in simultaneously inducing osteogenesis and angiogenesis, human bone marrow mesenchymal stem cells (hBMSCs) and human umblical vein endothelial cells (HUVECs) were cultured in scaffold extracts to investigate cell proliferation, viability, and expression of osteogenic and angiogenic genes.
The results showed that Mg-doped TCP scaffolds have the advantages of precise design, interconnected porous structure, and similar compressive strength to natural cancellous bone. hBMSCs and HUVECs exhibit high proliferation rate, cell morphology and viability in a certain amount of Mg
. In addition, this concentration of magnesium can also increase the expression levels of osteogenic and angiogenic biomarkers.
A certain concentration of magnesium ions plays an important role in new bone regeneration and reconstruction. It can be used as a simple and effective method to enhance the osteogenesis and angiogenesis of bioceramic scaffolds, and support the development of biomaterials and bone tissue engineering scaffolds. |
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AbstractList | Three-dimensional (3D) printed bone tissue engineering scaffolds have been widely used in research and clinical applications. β-TCP is a biomaterial commonly used in bone tissue engineering to treat bone defects, and its multifunctionality can be achieved by co-doping different metal ions. Magnesium doping in biomaterials has been shown to alter physicochemical properties of cells and enhance osteogenesis.
A series of Mg-doped TCP scaffolds were manufactured by using cryogenic 3D printing technology and sintering. The characteristics of the porous scaffolds, such as microstructure, chemical composition, mechanical properties, apparent porosity, etc., were examined. To further study the role of magnesium ions in simultaneously inducing osteogenesis and angiogenesis, human bone marrow mesenchymal stem cells (hBMSCs) and human umblical vein endothelial cells (HUVECs) were cultured in scaffold extracts to investigate cell proliferation, viability, and expression of osteogenic and angiogenic genes.
The results showed that Mg-doped TCP scaffolds have the advantages of precise design, interconnected porous structure, and similar compressive strength to natural cancellous bone. hBMSCs and HUVECs exhibit high proliferation rate, cell morphology and viability in a certain amount of Mg
. In addition, this concentration of magnesium can also increase the expression levels of osteogenic and angiogenic biomarkers.
A certain concentration of magnesium ions plays an important role in new bone regeneration and reconstruction. It can be used as a simple and effective method to enhance the osteogenesis and angiogenesis of bioceramic scaffolds, and support the development of biomaterials and bone tissue engineering scaffolds. |
Author | Liang, Guiping Gu, Yifan Zhang, Jing Niu, Wei Zhang, Xinzhi Xu, Tao |
Author_xml | – sequence: 1 givenname: Yifan surname: Gu fullname: Gu, Yifan organization: 2Orthopedics Department, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 111 Dade Road, Guangzhou, 510120 China – sequence: 2 givenname: Jing surname: Zhang fullname: Zhang, Jing organization: East China Institute of Digital Medical Engineering, Shangrao, 334000 China – sequence: 3 givenname: Xinzhi surname: Zhang fullname: Zhang, Xinzhi organization: East China Institute of Digital Medical Engineering, Shangrao, 334000 China – sequence: 4 givenname: Guiping surname: Liang fullname: Liang, Guiping organization: 2Orthopedics Department, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 111 Dade Road, Guangzhou, 510120 China – sequence: 5 givenname: Tao surname: Xu fullname: Xu, Tao organization: 6Department of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, Shenzhen, 518055 China – sequence: 6 givenname: Wei surname: Niu fullname: Niu, Wei organization: 2Orthopedics Department, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 111 Dade Road, Guangzhou, 510120 China |
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Snippet | Three-dimensional (3D) printed bone tissue engineering scaffolds have been widely used in research and clinical applications. β-TCP is a biomaterial commonly... |
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SubjectTerms | Biocompatible Materials - pharmacology Bone and Bones - drug effects Bone and Bones - metabolism Bone Regeneration - drug effects Calcium Phosphates - metabolism Cell Proliferation - drug effects Cells, Cultured Compressive Strength - drug effects Human Umbilical Vein Endothelial Cells - drug effects Human Umbilical Vein Endothelial Cells - metabolism Humans Ions - pharmacology Magnesium - pharmacology Mesenchymal Stem Cells - drug effects Mesenchymal Stem Cells - metabolism Neovascularization, Physiologic - drug effects Osteogenesis - drug effects Porosity - drug effects Printing, Three-Dimensional Tissue Engineering - methods Tissue Scaffolds - chemistry |
Title | Three-dimensional Printed Mg-Doped β-TCP Bone Tissue Engineering Scaffolds: Effects of Magnesium Ion Concentration on Osteogenesis and Angiogenesis In Vitro |
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