Nuclease-Resistant Ribozymes Decrease Stromelysin mRNA Levels in Rabbit Synovium Following Exogenous Delivery to the Knee Joint

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 93; no. 2; pp. 754 - 758
• Main Authors: Flory, Craig M., Pavco, Pamela A., Jarvis, Thale C., Lesch, Mark E., Wincott, Francine E., Beigelman, Leonid, Hunt, Stephen W., Schrier, Denis J.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 23.01.1996; National Acad Sciences; National Academy of Sciences
• Subjects: Animals; Arthritis; Arthritis - chemically induced; Arthritis - physiopathology; Base Sequence; Dose-Response Relationship, Drug; Gene Expression Regulation, Enzymologic; Genetics; Human resources; In Situ Hybridization; Injections, Intra-Articular; Interleukin-1 - pharmacology; Joint diseases; Knee; Knee joint; Knee Joint - drug effects; Male; Matrix Metalloproteinase 3; Medical treatment; Messenger RNA; Metalloendopeptidases - biosynthesis; Metalloendopeptidases - genetics; Molecular Sequence Data; Molecules; Proteoglycans; Rabbits; Ribonucleases - metabolism; Ribonucleic acid; RNA; RNA, Catalytic - metabolism; RNA, Catalytic - pharmacology; RNA, Messenger - biosynthesis; Synovial membrane; Synovial Membrane - drug effects

Catalytic RNA molecules, or ribozymes, have generated significant interest as potential therapeutic agents for controlling gene expression. Although ribozymes have been shown to work in vitro and in cellular assays, there are no reports that demonstrate the efficacy of synthetic, stabilized ribozyme...