Using terminal restriction fragment length polymorphism (T-RFLP) to identify mycorrhizal fungi: a methods review

• Published in: Mycorrhiza Vol. 17; no. 4; pp. 259 - 270
• Main Authors: Dickie, I. A, FitzJohn, R. G
• Format: Journal Article
• Language: English
• Published: Berlin Berlin/Heidelberg : Springer-Verlag 01.06.2007; Springer; Springer Nature B.V
• Subjects: Bacteria; Biological and medical sciences; classification; Cloning; Colony Count, Microbial; Databases, Genetic; DNA Primers; DNA Restriction Enzymes; DNA, Fungal; DNA, Fungal - genetics; DNA, Ribosomal Spacer; ectomycorrhizae; Fundamental and applied biological sciences. Psychology; Fungi; genetics; methods; Microbiology; Molecular identification; Mycological Typing Techniques; Mycological Typing Techniques - methods; mycorrhizae; Mycorrhizae - classification; Mycorrhizae - genetics; mycorrhizal fungi; Parasitism and symbiosis; Plant physiology and development; Polymerase Chain Reaction; Polymerase Chain Reaction - methods; Polymorphism, Restriction Fragment Length; polyphyly; restriction fragment length polymorphism; risk; species identification; Species Specificity; Symbiosis; Terminal restriction fragment length polymorphism (T-RFLP); vesicular arbuscular mycorrhizae; Fungi; Endomycorrhiza; Restriction fragment length polymorphism; Mycorrhiza; Ectomycorrhiza; Replication; Review; Arbuscular mycorrhiza; Molecular identification,Terminal restriction fragment length polymorphism (T-RFLP); Thallophyta

Terminal restriction fragment length polymorphism (T-RFLP) is an increasingly widely used technique in mycorrhizal ecology. In this paper, we review the technique as it is used to identify species of mycorrhizal fungi and distinguish two different versions of the technique: peak-profile T-RFLP (the...