Microsomal chitinase activity from Candida albicans

• Published in: Biochimica et biophysica acta Vol. 1073; no. 1; pp. 177 - 182
• Main Authors: Dickinson, Keith, Keer, Valerie, Hitchcock, Christopher A., Adams, David J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 23.01.1991; Elsevier
• Subjects: Acetylglucosamine; Acetylglucosamine - analogs & derivatives; Acetylglucosamine - pharmacology; analogs & derivatives; Analytical, structural and metabolic biochemistry; antagonists & inhibitors; Antifungal agent; Biological and medical sciences; C. albicans; Candida albicans; Candida albicans - enzymology; Cell Compartmentation; Centrifugation, Density Gradient; Chitin Synthase; Chitin Synthase - metabolism; chitinase; Chitinases; Chitinases - antagonists & inhibitors; Chitinases - metabolism; Cytosol; Cytosol - enzymology; enzyme activity; Enzyme kinetics; Enzymes and enzyme inhibitors; enzymology; Fundamental and applied biological sciences. Psychology; Hydrolases; kinetics; metabolism; Microsomal chitinase; microsomes; Microsomes - enzymology; pharmacology; purification; Solubility; Trisaccharides; Trypsin; Trypsin - pharmacology; Zymogen; Zymogen; Antifungal agent; Enzyme kinetics; C. albicans; Microsomal chitinase; Candida albicans; Chitinase; Detergent; Enzyme; Chitin synthase; Competitive inhibition; Microsome; Fungi; Enzymatic activity; Fungi Imperfecti; Kinetic parameter; Comparative study; Thallophyta

Chitinase (E.C. 3.2.1.14) was characterized in microsomal fractions from yeast cells of Candida albicans. Following six washes with buffer (50 mM Bis-Tris · Cl, pH 6.5), enzyme activity of microsomes fell markedly to 0.3% of total and 6% of the specific activity detected in the low-speed supernatant...