Peripheral Blood and Pleural Fluid Mononuclear Cell Responses to Low-Molecular-Mass Secretory Polypeptides of Mycobacterium tuberculosis in Human Models of Immunity to Tuberculosis

• Published in: Infection and Immunity Vol. 73; no. 6; pp. 3547 - 3558
• Main Authors: Sable, Suraj B, Kumar, Rajnish, Kalra, Mamta, Verma, Indu, Khuller, G. K, Dobos, Karen, Belisle, John T
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.06.2005
• Subjects: Adult; anion exchange; Antibodies, Bacterial; Antibodies, Bacterial - blood; antigens; Bacterial diseases; Bacterial Proteins; Bacterial Proteins - immunology; Biological and medical sciences; biosynthesis; blood; chromatography; Fundamental and applied biological sciences. Psychology; Host Response and Inflammation; Human bacterial diseases; Humans; immunity; immunology; Infectious diseases; Interferon-gamma; Interferon-gamma - biosynthesis; Leukocytes, Mononuclear; Leukocytes, Mononuclear - immunology; lymphocytes; Male; Medical sciences; Microbiology; Molecular Weight; Mycobacterium tuberculosis; patients; Pleural Effusion; Pleural Effusion - immunology; polyacrylamide gel electrophoresis; polypeptides; proteome; tuberculosis; Tuberculosis - immunology; Tuberculosis and atypical mycobacterial infections; vaccines; Human; Microbiology; Peripheral blood circulation; Mycobacterial infection; Immunity; Infection; Blood cell; Mononuclear cell; Tuberculosis; Mycobacterium tuberculosis; Mycobacteriales; Polypeptide; Bacteriosis; Mycobacteriaceae; Bacteria; Actinomycetes; Models
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.73.6.3547-3558.2005

A total of 104 polypeptides were purified from the low-molecular-mass secretory proteome of Mycobacterium tuberculosis H₃₇Rv using a combination of anion exchange column chromatography and high resolution preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroelution. The goal of this study was to identify polypeptides from a low-molecular-mass secretory proteome recognized by human subjects infected with M. tuberculosis and to ascertain the differences in specificity of antigen recognition by the peripheral blood mononuclear cells (PBMCs) and pleural fluid mononuclear cells (PFMCs) of these individuals. The study identified CFP-8 (Rv0496), CFP-11 (Rv2433c), CFP-14.5 (Rv2445c), and CFP-31 (Rv0831c) as novel T-cell antigens apart from previously characterized ESAT-6, TB10.4, CFP10, GroES, MTSP14, MTSP17, CFP21, MPT64, Ag85A, and Ag85B on the basis of recognition by PBMCs of tuberculosis contacts and treated tuberculosis patients. Further, polypeptides prominently recognized by PFMCs of tuberculous pleurisy patients were the same as those recognized by PBMCs of healthy contacts and treated tuberculosis patients. The results of our study indicate the homogeneity of antigenic target recognition by lymphocytes at the site of infection and at the periphery in the human subjects studied and the need to evaluate these antigenic targets as components of future antituberculous vaccines.