A Highly Verified Assay for KRAS Mutation Detection in Tissue and Plasma of Lung, Colorectal, and Pancreatic Cancer

• Published in: Archives of pathology & laboratory medicine (1976) Vol. 143; no. 2; pp. 183 - 189
• Main Authors: Li, Jing, Gan, Stephanie, Blair, Alan, Min, Kyungji, Rehage, Taraneh, Hoeppner, Corey, Halait, Harkanwal, Brophy, Victoria H.
• Format: Journal Article
• Language: English
• Published: United States College of American Pathologists 01.02.2019
• Subjects: Biochemical assays; Biomarkers; Body fluids; Cancer patients; Cancer screening; Clinical medicine; Colon cancer; Colorectal cancer; Colorectal Neoplasms - genetics; Deoxyribonucleic acid; Diagnosis; DNA; DNA Mutational Analysis - methods; EDTA; Formaldehyde; Gene mutation; Genes; Genetic aspects; Health aspects; Humans; Identification and classification; Initiatives; K-Ras protein; Laboratories; Lung cancer; Lung Neoplasms - genetics; Medical screening; Metastases; Metastasis; Mutation; Non-small cell lung cancer; Oncology; Operators; Pancreatic cancer; Pancreatic Neoplasms - genetics; Plasma; Proto-Oncogene Proteins p21(ras) - genetics; Real-Time Polymerase Chain Reaction - methods; Regulatory genes; Sensitivity and Specificity; Small cell lung cancer; Testing; Therapeutic applications; Tumors; Working groups

KRAS Mutation Test v2 is used for the qualitative detection and identification of 28 mutations in exons 2, 3, and 4 of the human KRAS gene. To verify the performance of KRAS Mutation Test v2 and to evaluate its accuracy by correlation with a next-generation sequencing method on Illumina MiSeq. In th...