Immunogenicity and functional characterization of Leishmania-derived hepatitis C virus envelope glycoprotein complex

Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 are the main inducers of a cross-neutralizing antibody response which plays an important role in the early phase of viral infection. Correctly folded and immunologically active E1E2 complex can be expressed in mammalian cells, though the produ...

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Published inScientific reports Vol. 6; no. 1; p. 30627
Main Authors Grzyb, Katarzyna, Czarnota, Anna, Brzozowska, Agnieszka, Cieślik, Anna, Rąbalski, Łukasz, Tyborowska, Jolanta, Bieńkowska-Szewczyk, Krystyna
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 02.08.2016
Nature Publishing Group
Subjects
13
13/1
13/109
631/250/255/234/2513/1551
631/61/24/590/2294
64
64/60
82/16
82/80
82/83
Animals
Antibodies, Neutralizing - blood
Antibody response
Cross Reactions
Cross-reactivity
Epitopes
Gene Expression
Genotypes
Glycoproteins
Hepacivirus - immunology
Hepatitis
Hepatitis C
Hepatitis C Antibodies - blood
Humanities and Social Sciences
Immune response
Immunogenicity
Leishmania - genetics
Mammalian cells
Mice
multidisciplinary
Recombinant Proteins - genetics
Recombinant Proteins - immunology
Science
Vaccines
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
Viral infections
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
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Abstract Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 are the main inducers of a cross-neutralizing antibody response which plays an important role in the early phase of viral infection. Correctly folded and immunologically active E1E2 complex can be expressed in mammalian cells, though the production process might still prove restrictive, even if the immunological response of a vaccine candidate is positive. Here, we report a characterization and immunogenicity study of a full-length (fE1E2) and soluble version of the E1E2 complex (tE1E2) from genotype 1a, successfully expressed in the cells of Leishmania tarentolae . In a functional study, we confirmed the binding of both Leishmania- derived E1E2 complexes to the CD-81 receptor and the presence of the major epitopes participating in a neutralizing antibody response. Both complexes were proved to be highly immunogenic in mice and elicited neutralizing antibody response. Moreover, cross-reactivity of the mouse sera was detected for all tested HCV genotypes with the highest signal intensity observed for genotypes 1a, 1b, 5 and 6. Since the development of a prophylactic vaccine against HCV is still needed to control the global infection, our Leishmania -derived E1E2 glycoproteins could be considered a potential cost-effective vaccine candidate.
AbstractList Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 are the main inducers of a cross-neutralizing antibody response which plays an important role in the early phase of viral infection. Correctly folded and immunologically active E1E2 complex can be expressed in mammalian cells, though the production process might still prove restrictive, even if the immunological response of a vaccine candidate is positive. Here, we report a characterization and immunogenicity study of a full-length (fE1E2) and soluble version of the E1E2 complex (tE1E2) from genotype 1a, successfully expressed in the cells of Leishmania tarentolae. In a functional study, we confirmed the binding of both Leishmania-derived E1E2 complexes to the CD-81 receptor and the presence of the major epitopes participating in a neutralizing antibody response. Both complexes were proved to be highly immunogenic in mice and elicited neutralizing antibody response. Moreover, cross-reactivity of the mouse sera was detected for all tested HCV genotypes with the highest signal intensity observed for genotypes 1a, 1b, 5 and 6. Since the development of a prophylactic vaccine against HCV is still needed to control the global infection, our Leishmania-derived E1E2 glycoproteins could be considered a potential cost-effective vaccine candidate.
Abstract Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 are the main inducers of a cross-neutralizing antibody response which plays an important role in the early phase of viral infection. Correctly folded and immunologically active E1E2 complex can be expressed in mammalian cells, though the production process might still prove restrictive, even if the immunological response of a vaccine candidate is positive. Here, we report a characterization and immunogenicity study of a full-length (fE1E2) and soluble version of the E1E2 complex (tE1E2) from genotype 1a, successfully expressed in the cells of Leishmania tarentolae . In a functional study, we confirmed the binding of both Leishmania- derived E1E2 complexes to the CD-81 receptor and the presence of the major epitopes participating in a neutralizing antibody response. Both complexes were proved to be highly immunogenic in mice and elicited neutralizing antibody response. Moreover, cross-reactivity of the mouse sera was detected for all tested HCV genotypes with the highest signal intensity observed for genotypes 1a, 1b, 5 and 6. Since the development of a prophylactic vaccine against HCV is still needed to control the global infection, our Leishmania -derived E1E2 glycoproteins could be considered a potential cost-effective vaccine candidate.
Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 are the main inducers of a cross-neutralizing antibody response which plays an important role in the early phase of viral infection. Correctly folded and immunologically active E1E2 complex can be expressed in mammalian cells, though the production process might still prove restrictive, even if the immunological response of a vaccine candidate is positive. Here, we report a characterization and immunogenicity study of a full-length (fE1E2) and soluble version of the E1E2 complex (tE1E2) from genotype 1a, successfully expressed in the cells of Leishmania tarentolae . In a functional study, we confirmed the binding of both Leishmania- derived E1E2 complexes to the CD-81 receptor and the presence of the major epitopes participating in a neutralizing antibody response. Both complexes were proved to be highly immunogenic in mice and elicited neutralizing antibody response. Moreover, cross-reactivity of the mouse sera was detected for all tested HCV genotypes with the highest signal intensity observed for genotypes 1a, 1b, 5 and 6. Since the development of a prophylactic vaccine against HCV is still needed to control the global infection, our Leishmania -derived E1E2 glycoproteins could be considered a potential cost-effective vaccine candidate.
ArticleNumber 30627
Author Rąbalski, Łukasz
Bieńkowska-Szewczyk, Krystyna
Czarnota, Anna
Brzozowska, Agnieszka
Grzyb, Katarzyna
Cieślik, Anna
Tyborowska, Jolanta
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Cites_doi 10.1111/j.1478-3231.2011.02684.x
10.1016/j.pep.2010.02.012
10.1107/S1744309110019330
10.1074/jbc.273.48.32088
10.1111/j.1600-065X.2010.00977.x
10.1371/journal.pone.0102235
10.1016/j.vaccine.2014.07.092
10.1016/S1046-5928(02)00001-3
10.3390/v6031149
10.1155/2013/450963
10.1002/hep.27013
10.1073/pnas.1114927109
10.1128/JVI.03574-13
10.1016/j.pep.2005.03.004
10.1099/vir.0.83386-0
10.3390/v7072809
10.1128/JVI.78.6.2994-3002.2004
10.1016/S1473-3099(05)70216-4
10.1093/glycob/3.3.193
10.1016/j.virol.2013.10.020
10.1128/JVI.79.21.13199-13208.2005
10.1371/journal.pone.0059776
10.1093/infdis/jir511
10.1073/pnas.0607026104
10.1128/JVI.01911-14
10.1128/JVI.75.22.11205-11217.2001
10.1371/journal.ppat.1000762
10.1016/j.jviromet.2015.11.006
10.3390/v3112127
10.1002/hep.26744
10.1128/JVI.05259-11
10.1371/journal.ppat.1002653
10.1056/NEJMoa1402454
10.1002/j.1460-2075.1995.tb07089.x
10.3389/fimmu.2014.00550
10.1126/science.1243876
10.1126/science.1114016
10.1099/vir.0.81995-0
10.1038/nm1268
10.1016/j.antiviral.2010.07.007
10.3390/v3101909
10.1128/JVI.01584-14
10.1128/JVI.01084-12
10.1128/JVI.78.3.1575-1581.2004
10.1016/j.pep.2009.07.005
10.1016/j.vaccine.2010.06.084
10.1016/j.jviromet.2013.05.018
10.1128/JVI.01941-12
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References Pestka (CR8) 2007; 104
Kushnir, Gase, Breitling, Alexandrov (CR22) 2005; 42
Op De Beeck (CR24) 2004; 78
Lange (CR48) 2014; 9
Breton, Zhao, Ouellette, Tremblay, Papadopoulou (CR33) 2007; 88
Pion (CR34) 2014; 32
Lanford (CR46) 2004; 78
Keck (CR45) 2013; 87
Helle, Duverlie, Dubuisson (CR37) 2011; 3
Keck (CR25) 2005; 79
Wong (CR15) 2014; 88
Wiese, Ilg, Lottspeich, Overath (CR23) 1995; 14
Cai (CR20) 2010; 88
Parodi (CR36) 1993; 3
CR49
Abdel-Hakeem, Shoukry (CR2) 2014; 5
Sautto, Tarr, Mancini, Clementi (CR44) 2013; 2013
Vieyres, Dubuisson, Pietschmann (CR39) 2014; 6
Shepard, Finelli, Alter (CR1) 2005; 5
Frey (CR12) 2010; 28
Keck (CR41) 2012; 8
Giang (CR18) 2012; 109
Smith (CR5) 2014; 59
Breitling (CR16) 2002; 25
Wang, Keck, Foung (CR9) 2011; 3
Tarr (CR7) 2015; 7
Phan, Sugino, Niimi (CR40) 2009; 68
Nakano, Lau, Lau, Sugiyama, Mizokami (CR6) 2012; 32
de Vries (CR38) 2012; 86
Kong (CR43) 2013; 342
Duvet (CR30) 1998; 273
Baechlein, Meemken, Pezzoni, Engemann, Grummer (CR32) 2013; 193
Tello (CR19) 2010; 71
Lindenbach (CR50) 2005; 309
Law (CR14) 2013; 8
Owsianka (CR42) 2008; 89
Wang (CR27) 2014; 448
Krey (CR29) 2010; 6
Osburn (CR10) 2014; 59
Keck (CR26) 2011; 85
Merola (CR31) 2001; 75
Afdhal (CR3) 2014; 370
Chmielewska (CR47) 2014; 88
Meunier (CR13) 2011; 204
Gazdag (CR17) 2010; 66
Cashman, Marsden, Dustin (CR4) 2014; 5
Houghton (CR11) 2011; 239
Lavie, Goffard, Dubuisson (CR28) 2007; 9
Fischer (CR35) 2015; 228
Ruwona, Giang, Nieusma, Law (CR21) 2014; 88
15947137 - Science. 2005 Jul 22;309(5734):623-6
21813602 - J Virol. 2011 Oct;85(20):10451-63
23527266 - PLoS One. 2013;8(3):e59776
24314653 - Virology. 2014 Jan 5;448:229-37
22163337 - Viruses. 2011 Nov;3(11):2127-45
24288331 - Science. 2013 Nov 29;342(6162):1090-4
17170454 - J Gen Virol. 2007 Jan;88(Pt 1):217-25
22511875 - PLoS Pathog. 2012;8(4):e1002653
14990718 - J Virol. 2004 Mar;78(6):2994-3002
19607924 - Protein Expr Purif. 2009 Nov;68(1):79-84
25275133 - J Virol. 2014 Dec;88(24):14278-88
24115039 - Hepatology. 2014 Jan;59(1):318-27
16227243 - J Virol. 2005 Nov;79(21):13199-208
25014219 - PLoS One. 2014 Jul 11;9(7):e102235
24982656 - Front Immunol. 2014 Jun 16;5:274
15939291 - Protein Expr Purif. 2005 Jul;42(1):37-46
26585033 - J Virol Methods. 2016 Feb;228:48-54
21198667 - Immunol Rev. 2011 Jan;239(1):99-108
23935648 - Clin Dev Immunol. 2013;2013:450963
8358146 - Glycobiology. 1993 Jun;3(3):193-9
22915811 - J Virol. 2012 Nov;86(21):11735-44
11602760 - J Virol. 2001 Nov;75(22):11205-17
24425349 - Hepatology. 2014 Jun;59(6):2140-51
23747546 - J Virol Methods. 2013 Oct;193(1):238-43
20619382 - Vaccine. 2010 Aug 31;28(38):6367-73
25131728 - Vaccine. 2014 Sep 29;32(43):5570-6
17392433 - Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):6025-30
14722311 - J Virol. 2004 Feb;78(3):1575-81
22142261 - Liver Int. 2012 Feb;32(2):339-45
20693657 - Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt 8):871-7
20691736 - Antiviral Res. 2010 Oct;88(1):80-5
18272755 - J Gen Virol. 2008 Mar;89(Pt 3):653-9
24965471 - J Virol. 2014 Sep;88(18):10459-71
9822684 - J Biol Chem. 1998 Nov 27;273(48):32088-95
25426115 - Front Immunol. 2014 Nov 10;5:550
24618856 - Viruses. 2014 Mar 11;6(3):1149-87
24599994 - J Virol. 2014 May;88(10):5502-10
12135552 - Protein Expr Purif. 2002 Jul;25(2):209-18
22069522 - Viruses. 2011 Oct;3(10):1909-32
20174556 - PLoS Pathog. 2010 Feb 19;6(2):e1000762
21917891 - J Infect Dis. 2011 Oct 15;204(8):1186-90
23097455 - J Virol. 2013 Jan;87(1):37-51
17489436 - Curr Issues Mol Biol. 2007 Jul;9(2):71-86
26193307 - Viruses. 2015 Jul 17;7(7):3995-4046
7720697 - EMBO J. 1995 Mar 15;14(6):1067-74
20176110 - Protein Expr Purif. 2010 Jun;71(2):123-31
16122679 - Lancet Infect Dis. 2005 Sep;5(9):558-67
15951748 - Nat Med. 2005 Jul;11(7):791-6
24725239 - N Engl J Med. 2014 May 15;370(20):1889-98
22492964 - Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6205-10
Z Keck (BFsrep30627_CR41) 2012; 8
F Helle (BFsrep30627_CR37) 2011; 3
C Baechlein (BFsrep30627_CR32) 2013; 193
AJ Parodi (BFsrep30627_CR36) 1993; 3
DB Smith (BFsrep30627_CR5) 2014; 59
M Lavie (BFsrep30627_CR28) 2007; 9
Z-Y Keck (BFsrep30627_CR26) 2011; 85
BD Lindenbach (BFsrep30627_CR50) 2005; 309
JaJ-X Wong (BFsrep30627_CR15) 2014; 88
C Pion (BFsrep30627_CR34) 2014; 32
Z-Y Keck (BFsrep30627_CR25) 2005; 79
Z Keck (BFsrep30627_CR45) 2013; 87
R Breitling (BFsrep30627_CR16) 2002; 25
J-C Meunier (BFsrep30627_CR13) 2011; 204
G Vieyres (BFsrep30627_CR39) 2014; 6
JM Pestka (BFsrep30627_CR8) 2007; 104
JLM Law (BFsrep30627_CR14) 2013; 8
M Lange (BFsrep30627_CR48) 2014; 9
W Cai (BFsrep30627_CR20) 2010; 88
BFsrep30627_CR49
AW Tarr (BFsrep30627_CR7) 2015; 7
TB Ruwona (BFsrep30627_CR21) 2014; 88
SE Frey (BFsrep30627_CR12) 2010; 28
W Wang (BFsrep30627_CR27) 2014; 448
RP de Vries (BFsrep30627_CR38) 2012; 86
EM Gazdag (BFsrep30627_CR17) 2010; 66
MS Abdel-Hakeem (BFsrep30627_CR2) 2014; 5
WO Osburn (BFsrep30627_CR10) 2014; 59
M Wiese (BFsrep30627_CR23) 1995; 14
M Merola (BFsrep30627_CR31) 2001; 75
AM Owsianka (BFsrep30627_CR42) 2008; 89
S Duvet (BFsrep30627_CR30) 1998; 273
T Krey (BFsrep30627_CR29) 2010; 6
M Houghton (BFsrep30627_CR11) 2011; 239
E Giang (BFsrep30627_CR18) 2012; 109
T Nakano (BFsrep30627_CR6) 2012; 32
G Sautto (BFsrep30627_CR44) 2013; 2013
K Fischer (BFsrep30627_CR35) 2015; 228
Y Wang (BFsrep30627_CR9) 2011; 3
CW Shepard (BFsrep30627_CR1) 2005; 5
H-P Phan (BFsrep30627_CR40) 2009; 68
RE Lanford (BFsrep30627_CR46) 2004; 78
M Breton (BFsrep30627_CR33) 2007; 88
N Afdhal (BFsrep30627_CR3) 2014; 370
AM Chmielewska (BFsrep30627_CR47) 2014; 88
D Tello (BFsrep30627_CR19) 2010; 71
L Kong (BFsrep30627_CR43) 2013; 342
SB Cashman (BFsrep30627_CR4) 2014; 5
A Op De Beeck (BFsrep30627_CR24) 2004; 78
S Kushnir (BFsrep30627_CR22) 2005; 42
References_xml – volume: 6
  start-page: e1000762
  year: 2010
  ident: CR29
  article-title: The Disulfide Bonds in Glycoprotein E2 of Hepatitis C Virus Reveal the Tertiary Organization of the Molecule
  publication-title: PLoS Pathog.
  contributor:
    fullname: Krey
– volume: 68
  start-page: 79
  year: 2009
  end-page: 84
  ident: CR40
  article-title: The production of recombinant human laminin-332 in a Leishmania tarentolae expression system
  publication-title: Protein Expr. Purif.
  contributor:
    fullname: Niimi
– ident: CR49
– volume: 71
  start-page: 123
  year: 2010
  end-page: 131
  ident: CR19
  article-title: Expression and structural properties of a chimeric protein based on the ectodomains of E1 and E2 hepatitis C virus envelope glycoproteins
  publication-title: Protein Expr. Purif.
  contributor:
    fullname: Tello
– volume: 228
  start-page: 48
  year: 2015
  end-page: 54
  ident: CR35
  article-title: Expression, characterisation and antigenicity of a truncated Hendra virus attachment protein expressed in the protozoan host Leishmania tarentolae
  publication-title: J. Virol. Methods
  contributor:
    fullname: Fischer
– volume: 14
  start-page: 1067
  year: 1995
  end-page: 1074
  ident: CR23
  article-title: Ser/Thr-rich repetitive motifs as targets for phosphoglycan modifications in Leishmania mexicana secreted acid phosphatase
  publication-title: EMBO J.
  contributor:
    fullname: Overath
– volume: 9
  start-page: e102235
  year: 2014
  ident: CR48
  article-title: Hepatitis C Virus Hypervariable Region 1 Variants Presented on Hepatitis B Virus Capsid-Like Particles Induce Cross-Neutralizing Antibodies
  publication-title: PLoS One
  contributor:
    fullname: Lange
– volume: 7
  start-page: 3995
  year: 2015
  end-page: 4046
  ident: CR7
  article-title: Genetic Diversity Underlying the Envelope Glycoproteins of Hepatitis C Virus: Structural and Functional Consequences and the Implications for Vaccine Design
  publication-title: Viruses
  contributor:
    fullname: Tarr
– volume: 8
  start-page: e1002653
  year: 2012
  ident: CR41
  article-title: Human monoclonal antibodies to a novel cluster of conformational epitopes on HCV E2 with resistance to neutralization escape in a genotype 2a isolate
  publication-title: PLoS Pathog.
  contributor:
    fullname: Keck
– volume: 239
  start-page: 99
  year: 2011
  end-page: 108
  ident: CR11
  article-title: Prospects for prophylactic and therapeutic vaccines against the hepatitis C viruses
  publication-title: Immunol. Rev.
  contributor:
    fullname: Houghton
– volume: 6
  start-page: 1149
  year: 2014
  end-page: 1187
  ident: CR39
  article-title: Incorporation of hepatitis C virus E1 and E2 glycoproteins: the keystones on a peculiar virion
  publication-title: Viruses
  contributor:
    fullname: Pietschmann
– volume: 370
  start-page: 1889
  year: 2014
  end-page: 1898
  ident: CR3
  article-title: Ledipasvir and sofosbuvir for untreated HCV genotype 1 infection
  publication-title: N. Engl. J. Med.
  contributor:
    fullname: Afdhal
– volume: 59
  start-page: 2140
  year: 2014
  end-page: 2151
  ident: CR10
  article-title: Clearance of hepatitis C infection is associated with the early appearance of broad neutralizing antibody responses
  publication-title: Hepatology
  contributor:
    fullname: Osburn
– volume: 25
  start-page: 209
  year: 2002
  end-page: 218
  ident: CR16
  article-title: Non-pathogenic trypanosomatid protozoa as a platform for protein research and production
  publication-title: Protein Expr. Purif.
  contributor:
    fullname: Breitling
– volume: 88
  start-page: 80
  year: 2010
  end-page: 85
  ident: CR20
  article-title: Expression, purification and immunogenic characterization of hepatitis C virus recombinant E1E2 protein expressed by Pichia pastoris yeast
  publication-title: Antiviral Res.
  contributor:
    fullname: Cai
– volume: 88
  start-page: 217
  year: 2007
  end-page: 225
  ident: CR33
  article-title: A recombinant non-pathogenic Leishmania vaccine expressing human immunodeficiency virus 1 (HIV-1) Gag elicits cell-mediated immunity in mice and decreases HIV-1 replication in human tonsillar tissue following exposure to HIV-1 infection
  publication-title: J. Gen. Virol.
  contributor:
    fullname: Papadopoulou
– volume: 87
  start-page: 37
  year: 2013
  end-page: 51
  ident: CR45
  article-title: Cooperativity in virus neutralization by human monoclonal antibodies to two adjacent regions located at the amino terminus of hepatitis C virus E2 glycoprotein
  publication-title: J. Virol.
  contributor:
    fullname: Keck
– volume: 42
  start-page: 37
  year: 2005
  end-page: 46
  ident: CR22
  article-title: Development of an inducible protein expression system based on the protozoan host Leishmania tarentolae
  publication-title: Protein Expr. Purif.
  contributor:
    fullname: Alexandrov
– volume: 342
  start-page: 1090
  year: 2013
  end-page: 1094
  ident: CR43
  article-title: Hepatitis C virus E2 envelope glycoprotein core structure
  publication-title: Science
  contributor:
    fullname: Kong
– volume: 88
  start-page: 10459
  year: 2014
  end-page: 10471
  ident: CR21
  article-title: Fine mapping of murine antibody responses to immunization with a novel soluble form of hepatitis C virus envelope glycoprotein complex
  publication-title: J. Virol.
  contributor:
    fullname: Law
– volume: 109
  start-page: 6205
  year: 2012
  end-page: 6210
  ident: CR18
  article-title: Human broadly neutralizing antibodies to the envelope glycoprotein complex of hepatitis C virus
  publication-title: Proc. Natl. Acad. Sci. USA.
  contributor:
    fullname: Giang
– volume: 5
  start-page: 550
  year: 2014
  ident: CR4
  article-title: The Humoral Immune Response to HCV: Understanding is Key to Vaccine Development
  publication-title: Front. Immunol.
  contributor:
    fullname: Dustin
– volume: 32
  start-page: 5570
  year: 2014
  end-page: 5576
  ident: CR34
  article-title: Characterization and immunogenicity in mice of recombinant influenza haemagglutinins produced in Leishmania tarentolae
  publication-title: Vaccine
  contributor:
    fullname: Pion
– volume: 204
  start-page: 1186
  year: 2011
  end-page: 1190
  ident: CR13
  article-title: Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus
  publication-title: J. Infect. Dis.
  contributor:
    fullname: Meunier
– volume: 28
  start-page: 6367
  year: 2010
  end-page: 6373
  ident: CR12
  article-title: Safety and immunogenicity of HCV E1E2 vaccine adjuvanted with MF59 administered to healthy adults
  publication-title: Vaccine
  contributor:
    fullname: Frey
– volume: 2013
  start-page: 450963
  year: 2013
  ident: CR44
  article-title: Structural and antigenic definition of hepatitis C virus E2 glycoprotein epitopes targeted by monoclonal antibodies
  publication-title: Clin. Dev. Immunol.
  contributor:
    fullname: Clementi
– volume: 75
  start-page: 11205
  year: 2001
  end-page: 11217
  ident: CR31
  article-title: Folding of Hepatitis C Virus E1 Glycoprotein in a Cell-Free System
  publication-title: J. Virol.
  contributor:
    fullname: Merola
– volume: 3
  start-page: 193
  year: 1993
  end-page: 199
  ident: CR36
  article-title: N-glycosylation in trypanosomatid protozoa
  publication-title: Glycobiology
  contributor:
    fullname: Parodi
– volume: 5
  start-page: 274
  year: 2014
  ident: CR2
  article-title: Protective immunity against hepatitis C: many shades of gray
  publication-title: Front. Immunol
  contributor:
    fullname: Shoukry
– volume: 3
  start-page: 2127
  year: 2011
  end-page: 2145
  ident: CR9
  article-title: Neutralizing antibody response to hepatitis C virus
  publication-title: Viruses
  contributor:
    fullname: Foung
– volume: 8
  start-page: e59776
  year: 2013
  ident: CR14
  article-title: A hepatitis C virus (HCV) vaccine comprising envelope glycoproteins gpE1/gpE2 derived from a single isolate elicits broad cross-genotype neutralizing antibodies in humans
  publication-title: PLoS One
  contributor:
    fullname: Law
– volume: 86
  start-page: 11735
  year: 2012
  end-page: 11744
  ident: CR38
  article-title: Glycan-dependent immunogenicity of recombinant soluble trimeric hemagglutinin
  publication-title: J. Virol.
  contributor:
    fullname: de Vries
– volume: 85
  start-page: 10451
  year: 2011
  end-page: 10463
  ident: CR26
  article-title: Mapping a region of hepatitis C virus E2 that is responsible for escape from neutralizing antibodies and a core CD81-binding region that does not tolerate neutralization escape mutations
  publication-title: J. Virol.
  contributor:
    fullname: Keck
– volume: 66
  start-page: 871
  year: 2010
  end-page: 877
  ident: CR17
  article-title: Purification and crystallization of human Cu/Zn superoxide dismutase recombinantly produced in the protozoan Leishmania tarentolae
  publication-title: Acta Crystallogr. Sect. F. Struct. Biol. Cryst. Commun
  contributor:
    fullname: Gazdag
– volume: 88
  start-page: 5502
  year: 2014
  end-page: 5510
  ident: CR47
  article-title: Combined Adenovirus Vector and Hepatitis C Virus Envelope Protein Prime-Boost Regimen Elicits T Cell and Neutralizing Antibody Immune Responses
  publication-title: J. Virol.
  contributor:
    fullname: Chmielewska
– volume: 3
  start-page: 1909
  year: 2011
  end-page: 1932
  ident: CR37
  article-title: The hepatitis C virus glycan shield and evasion of the humoral immune response
  publication-title: Viruses
  contributor:
    fullname: Dubuisson
– volume: 88
  start-page: 14278
  year: 2014
  end-page: 14288
  ident: CR15
  article-title: Recombinant Hepatitis C Virus Envelope Glycoprotein Vaccine Elicits Antibodies Targeting Multiple Epitopes on the Envelope Glycoproteins Associated with Broad Cross-Neutralization
  publication-title: J. Virol.
  contributor:
    fullname: Wong
– volume: 5
  start-page: 558
  year: 2005
  end-page: 567
  ident: CR1
  article-title: Global epidemiology of hepatitis C virus infection
  publication-title: Lancet Infect. Dis.
  contributor:
    fullname: Alter
– volume: 193
  start-page: 238
  year: 2013
  end-page: 243
  ident: CR32
  article-title: Expression of a truncated hepatitis E virus capsid protein in the protozoan organism Leishmania tarentolae and its application in a serological assay
  publication-title: J. Virol. Methods
  contributor:
    fullname: Grummer
– volume: 104
  start-page: 6025
  year: 2007
  end-page: 6030
  ident: CR8
  article-title: Rapid induction of virus-neutralizing antibodies and viral clearance in a single-source outbreak of hepatitis C
  publication-title: Proc. Natl. Acad. Sci. USA
  contributor:
    fullname: Pestka
– volume: 59
  start-page: 318
  year: 2014
  end-page: 327
  ident: CR5
  article-title: Expanded classification of hepatitis C virus into 7 genotypes and 67 subtypes: updated criteria and genotype assignment web resource
  publication-title: Hepatology
  contributor:
    fullname: Smith
– volume: 32
  start-page: 339
  year: 2012
  end-page: 345
  ident: CR6
  article-title: An updated analysis of hepatitis C virus genotypes and subtypes based on the complete coding region
  publication-title: Liver Int.
  contributor:
    fullname: Mizokami
– volume: 78
  start-page: 2994
  year: 2004
  end-page: 3002
  ident: CR24
  article-title: Characterization of functional hepatitis C virus envelope glycoproteins
  publication-title: J. Virol.
  contributor:
    fullname: Op De Beeck
– volume: 309
  start-page: 623
  year: 2005
  end-page: 626
  ident: CR50
  article-title: Complete replication of hepatitis C virus in cell culture
  publication-title: Science
  contributor:
    fullname: Lindenbach
– volume: 78
  start-page: 1575
  year: 2004
  end-page: 1581
  ident: CR46
  article-title: Cross-genotype immunity to hepatitis C virus
  publication-title: J. Virol.
  contributor:
    fullname: Lanford
– volume: 79
  start-page: 13199
  year: 2005
  end-page: 13208
  ident: CR25
  article-title: Analysis of a highly flexible conformational immunogenic domain a in hepatitis C virus E2
  publication-title: J. Virol.
  contributor:
    fullname: Keck
– volume: 9
  start-page: 71
  year: 2007
  end-page: 86
  ident: CR28
  article-title: Assembly of a functional HCV glycoprotein heterodimer
  publication-title: Curr. Issues Mol. Biol.
  contributor:
    fullname: Dubuisson
– volume: 448
  start-page: 229
  year: 2014
  end-page: 237
  ident: CR27
  article-title: Alanine scanning mutagenesis of hepatitis C virus E2 cysteine residues: Insights into E2 biogenesis and antigenicity
  publication-title: Virology
  contributor:
    fullname: Wang
– volume: 89
  start-page: 653
  year: 2008
  end-page: 659
  ident: CR42
  article-title: Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein
  publication-title: J. Gen. Virol.
  contributor:
    fullname: Owsianka
– volume: 273
  start-page: 32088
  year: 1998
  end-page: 32095
  ident: CR30
  article-title: Hepatitis C virus glycoprotein complex localization in the endoplasmic reticulum involves a determinant for retention and not retrieval
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Duvet
– volume: 32
  start-page: 339
  year: 2012
  ident: BFsrep30627_CR6
  publication-title: Liver Int.
  doi: 10.1111/j.1478-3231.2011.02684.x
  contributor:
    fullname: T Nakano
– volume: 71
  start-page: 123
  year: 2010
  ident: BFsrep30627_CR19
  publication-title: Protein Expr. Purif.
  doi: 10.1016/j.pep.2010.02.012
  contributor:
    fullname: D Tello
– volume: 66
  start-page: 871
  year: 2010
  ident: BFsrep30627_CR17
  publication-title: Acta Crystallogr. Sect. F. Struct. Biol. Cryst. Commun
  doi: 10.1107/S1744309110019330
  contributor:
    fullname: EM Gazdag
– volume: 273
  start-page: 32088
  year: 1998
  ident: BFsrep30627_CR30
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.273.48.32088
  contributor:
    fullname: S Duvet
– volume: 239
  start-page: 99
  year: 2011
  ident: BFsrep30627_CR11
  publication-title: Immunol. Rev.
  doi: 10.1111/j.1600-065X.2010.00977.x
  contributor:
    fullname: M Houghton
– volume: 9
  start-page: e102235
  year: 2014
  ident: BFsrep30627_CR48
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0102235
  contributor:
    fullname: M Lange
– volume: 32
  start-page: 5570
  year: 2014
  ident: BFsrep30627_CR34
  publication-title: Vaccine
  doi: 10.1016/j.vaccine.2014.07.092
  contributor:
    fullname: C Pion
– volume: 25
  start-page: 209
  year: 2002
  ident: BFsrep30627_CR16
  publication-title: Protein Expr. Purif.
  doi: 10.1016/S1046-5928(02)00001-3
  contributor:
    fullname: R Breitling
– volume: 6
  start-page: 1149
  year: 2014
  ident: BFsrep30627_CR39
  publication-title: Viruses
  doi: 10.3390/v6031149
  contributor:
    fullname: G Vieyres
– volume: 2013
  start-page: 450963
  year: 2013
  ident: BFsrep30627_CR44
  publication-title: Clin. Dev. Immunol.
  doi: 10.1155/2013/450963
  contributor:
    fullname: G Sautto
– volume: 59
  start-page: 2140
  year: 2014
  ident: BFsrep30627_CR10
  publication-title: Hepatology
  doi: 10.1002/hep.27013
  contributor:
    fullname: WO Osburn
– volume: 109
  start-page: 6205
  year: 2012
  ident: BFsrep30627_CR18
  publication-title: Proc. Natl. Acad. Sci. USA.
  doi: 10.1073/pnas.1114927109
  contributor:
    fullname: E Giang
– volume: 88
  start-page: 5502
  year: 2014
  ident: BFsrep30627_CR47
  publication-title: J. Virol.
  doi: 10.1128/JVI.03574-13
  contributor:
    fullname: AM Chmielewska
– volume: 9
  start-page: 71
  year: 2007
  ident: BFsrep30627_CR28
  publication-title: Curr. Issues Mol. Biol.
  contributor:
    fullname: M Lavie
– volume: 42
  start-page: 37
  year: 2005
  ident: BFsrep30627_CR22
  publication-title: Protein Expr. Purif.
  doi: 10.1016/j.pep.2005.03.004
  contributor:
    fullname: S Kushnir
– volume: 89
  start-page: 653
  year: 2008
  ident: BFsrep30627_CR42
  publication-title: J. Gen. Virol.
  doi: 10.1099/vir.0.83386-0
  contributor:
    fullname: AM Owsianka
– volume: 7
  start-page: 3995
  year: 2015
  ident: BFsrep30627_CR7
  publication-title: Viruses
  doi: 10.3390/v7072809
  contributor:
    fullname: AW Tarr
– volume: 78
  start-page: 2994
  year: 2004
  ident: BFsrep30627_CR24
  publication-title: J. Virol.
  doi: 10.1128/JVI.78.6.2994-3002.2004
  contributor:
    fullname: A Op De Beeck
– volume: 5
  start-page: 558
  year: 2005
  ident: BFsrep30627_CR1
  publication-title: Lancet Infect. Dis.
  doi: 10.1016/S1473-3099(05)70216-4
  contributor:
    fullname: CW Shepard
– volume: 3
  start-page: 193
  year: 1993
  ident: BFsrep30627_CR36
  publication-title: Glycobiology
  doi: 10.1093/glycob/3.3.193
  contributor:
    fullname: AJ Parodi
– volume: 448
  start-page: 229
  year: 2014
  ident: BFsrep30627_CR27
  publication-title: Virology
  doi: 10.1016/j.virol.2013.10.020
  contributor:
    fullname: W Wang
– volume: 79
  start-page: 13199
  year: 2005
  ident: BFsrep30627_CR25
  publication-title: J. Virol.
  doi: 10.1128/JVI.79.21.13199-13208.2005
  contributor:
    fullname: Z-Y Keck
– volume: 8
  start-page: e59776
  year: 2013
  ident: BFsrep30627_CR14
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0059776
  contributor:
    fullname: JLM Law
– volume: 204
  start-page: 1186
  year: 2011
  ident: BFsrep30627_CR13
  publication-title: J. Infect. Dis.
  doi: 10.1093/infdis/jir511
  contributor:
    fullname: J-C Meunier
– volume: 104
  start-page: 6025
  year: 2007
  ident: BFsrep30627_CR8
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0607026104
  contributor:
    fullname: JM Pestka
– volume: 88
  start-page: 14278
  year: 2014
  ident: BFsrep30627_CR15
  publication-title: J. Virol.
  doi: 10.1128/JVI.01911-14
  contributor:
    fullname: JaJ-X Wong
– volume: 75
  start-page: 11205
  year: 2001
  ident: BFsrep30627_CR31
  publication-title: J. Virol.
  doi: 10.1128/JVI.75.22.11205-11217.2001
  contributor:
    fullname: M Merola
– volume: 6
  start-page: e1000762
  year: 2010
  ident: BFsrep30627_CR29
  publication-title: PLoS Pathog.
  doi: 10.1371/journal.ppat.1000762
  contributor:
    fullname: T Krey
– volume: 228
  start-page: 48
  year: 2015
  ident: BFsrep30627_CR35
  publication-title: J. Virol. Methods
  doi: 10.1016/j.jviromet.2015.11.006
  contributor:
    fullname: K Fischer
– volume: 3
  start-page: 2127
  year: 2011
  ident: BFsrep30627_CR9
  publication-title: Viruses
  doi: 10.3390/v3112127
  contributor:
    fullname: Y Wang
– volume: 59
  start-page: 318
  year: 2014
  ident: BFsrep30627_CR5
  publication-title: Hepatology
  doi: 10.1002/hep.26744
  contributor:
    fullname: DB Smith
– volume: 85
  start-page: 10451
  year: 2011
  ident: BFsrep30627_CR26
  publication-title: J. Virol.
  doi: 10.1128/JVI.05259-11
  contributor:
    fullname: Z-Y Keck
– volume: 8
  start-page: e1002653
  year: 2012
  ident: BFsrep30627_CR41
  publication-title: PLoS Pathog.
  doi: 10.1371/journal.ppat.1002653
  contributor:
    fullname: Z Keck
– volume: 370
  start-page: 1889
  year: 2014
  ident: BFsrep30627_CR3
  publication-title: N. Engl. J. Med.
  doi: 10.1056/NEJMoa1402454
  contributor:
    fullname: N Afdhal
– volume: 14
  start-page: 1067
  year: 1995
  ident: BFsrep30627_CR23
  publication-title: EMBO J.
  doi: 10.1002/j.1460-2075.1995.tb07089.x
  contributor:
    fullname: M Wiese
– volume: 5
  start-page: 550
  year: 2014
  ident: BFsrep30627_CR4
  publication-title: Front. Immunol.
  doi: 10.3389/fimmu.2014.00550
  contributor:
    fullname: SB Cashman
– volume: 342
  start-page: 1090
  year: 2013
  ident: BFsrep30627_CR43
  publication-title: Science
  doi: 10.1126/science.1243876
  contributor:
    fullname: L Kong
– volume: 309
  start-page: 623
  year: 2005
  ident: BFsrep30627_CR50
  publication-title: Science
  doi: 10.1126/science.1114016
  contributor:
    fullname: BD Lindenbach
– volume: 88
  start-page: 217
  year: 2007
  ident: BFsrep30627_CR33
  publication-title: J. Gen. Virol.
  doi: 10.1099/vir.0.81995-0
  contributor:
    fullname: M Breton
– ident: BFsrep30627_CR49
  doi: 10.1038/nm1268
– volume: 5
  start-page: 274
  year: 2014
  ident: BFsrep30627_CR2
  publication-title: Front. Immunol
  contributor:
    fullname: MS Abdel-Hakeem
– volume: 88
  start-page: 80
  year: 2010
  ident: BFsrep30627_CR20
  publication-title: Antiviral Res.
  doi: 10.1016/j.antiviral.2010.07.007
  contributor:
    fullname: W Cai
– volume: 3
  start-page: 1909
  year: 2011
  ident: BFsrep30627_CR37
  publication-title: Viruses
  doi: 10.3390/v3101909
  contributor:
    fullname: F Helle
– volume: 88
  start-page: 10459
  year: 2014
  ident: BFsrep30627_CR21
  publication-title: J. Virol.
  doi: 10.1128/JVI.01584-14
  contributor:
    fullname: TB Ruwona
– volume: 86
  start-page: 11735
  year: 2012
  ident: BFsrep30627_CR38
  publication-title: J. Virol.
  doi: 10.1128/JVI.01084-12
  contributor:
    fullname: RP de Vries
– volume: 78
  start-page: 1575
  year: 2004
  ident: BFsrep30627_CR46
  publication-title: J. Virol.
  doi: 10.1128/JVI.78.3.1575-1581.2004
  contributor:
    fullname: RE Lanford
– volume: 68
  start-page: 79
  year: 2009
  ident: BFsrep30627_CR40
  publication-title: Protein Expr. Purif.
  doi: 10.1016/j.pep.2009.07.005
  contributor:
    fullname: H-P Phan
– volume: 28
  start-page: 6367
  year: 2010
  ident: BFsrep30627_CR12
  publication-title: Vaccine
  doi: 10.1016/j.vaccine.2010.06.084
  contributor:
    fullname: SE Frey
– volume: 193
  start-page: 238
  year: 2013
  ident: BFsrep30627_CR32
  publication-title: J. Virol. Methods
  doi: 10.1016/j.jviromet.2013.05.018
  contributor:
    fullname: C Baechlein
– volume: 87
  start-page: 37
  year: 2013
  ident: BFsrep30627_CR45
  publication-title: J. Virol.
  doi: 10.1128/JVI.01941-12
  contributor:
    fullname: Z Keck
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Snippet Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 are the main inducers of a cross-neutralizing antibody response which plays an important role in the...
Abstract Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 are the main inducers of a cross-neutralizing antibody response which plays an important role...
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631/61/24/590/2294
64
64/60
82/16
82/80
82/83
Animals
Antibodies, Neutralizing - blood
Antibody response
Cross Reactions
Cross-reactivity
Epitopes
Gene Expression
Genotypes
Glycoproteins
Hepacivirus - immunology
Hepatitis
Hepatitis C
Hepatitis C Antibodies - blood
Humanities and Social Sciences
Immune response
Immunogenicity
Leishmania - genetics
Mammalian cells
Mice
multidisciplinary
Recombinant Proteins - genetics
Recombinant Proteins - immunology
Science
Vaccines
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
Viral infections
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
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Title Immunogenicity and functional characterization of Leishmania-derived hepatitis C virus envelope glycoprotein complex
URI https://link.springer.com/article/10.1038/srep30627
https://www.ncbi.nlm.nih.gov/pubmed/27481352
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Volume 6
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