Development of high-yield influenza A virus vaccine viruses

• Published in: Nature communications Vol. 6; no. 1; p. 8148
• Main Authors: Ping, Jihui, Lopes, Tiago J.S., Nidom, Chairul A., Ghedin, Elodie, Macken, Catherine A., Fitch, Adam, Imai, Masaki, Maher, Eileen A., Neumann, Gabriele, Kawaoka, Yoshihiro
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02.09.2015; Nature Publishing Group; Nature Pub. Group
• Subjects: 13/106; 13/109; 38/23; 42/40; 631/154/309/2144; 631/250/24/590; 631/326/596/1578; 64/60; Animals; Backbone; Base Sequence; Cell culture; Cercopithecus aethiops; Dogs; Eggs; Exo-a-sialidase; HEK293 Cells; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinins; Humanities and Social Sciences; Humans; Influenza; Influenza A; Influenza A virus - genetics; Influenza A virus - immunology; Influenza A Virus, H1N1 Subtype - genetics; Influenza A Virus, H1N1 Subtype - immunology; Influenza A Virus, H3N2 Subtype - genetics; Influenza A Virus, H3N2 Subtype - immunology; Influenza A Virus, H5N1 Subtype - genetics; Influenza A Virus, H5N1 Subtype - immunology; Influenza A Virus, H7N9 Subtype - genetics; Influenza A Virus, H7N9 Subtype - immunology; Influenza Vaccines - biosynthesis; Influenza Vaccines - immunology; Kidneys; Madin Darby Canine Kidney Cells; Molecular Sequence Data; multidisciplinary; Mutation; Neuraminidase - genetics; Pandemics; Propagation; Regulatory sequences; Ribonucleic acid; RNA; Science; Science (multidisciplinary); Screens; Vaccine development; Vaccines; Vero Cells; Viruses

Vaccination is one of the most cost-effective ways to prevent infection. Influenza vaccines propagated in cultured cells are approved for use in humans, but their yields are often suboptimal. Here, we screened A/Puerto Rico/8/34 (PR8) virus mutant libraries to develop vaccine backbones (defined here...