Two Pathways of Sphingolipid Biosynthesis Are Separated in the Yeast Pichia pastoris

• Published in: The Journal of biological chemistry Vol. 286; no. 13; pp. 11401 - 11414
• Main Authors: Ternes, Philipp, Wobbe, Tobias, Schwarz, Marnie, Albrecht, Sandra, Feussner, Kirstin, Riezman, Isabelle, Cregg, James M., Heinz, Ernst, Riezman, Howard, Feussner, Ivo, Warnecke, Dirk
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2011; American Society for Biochemistry and Molecular Biology
• Subjects: Biosynthetic Pathway; Ceramide; Ceramide Synthase; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene Knock-out; Genes, Fungal - physiology; Glucosylceramide; Glycolipid Structure; Glycolipids; Lipid; Lipids; Mass Spectrometry (MS); Oxidoreductases - genetics; Oxidoreductases - metabolism; Pichia - genetics; Pichia - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Species Specificity; Sphingolipid; Sphingolipids - biosynthesis; Sphingolipids - genetics; Glycolipid Structure; Glycolipids; Gene Knock-out; Mass Spectrometry (MS); Ceramide; Sphingolipid; Biosynthetic Pathway; Ceramide Synthase; Glucosylceramide; Lipid

Although the yeast Saccharomyces cerevisiae has only one sphingolipid class with a head group based on phosphoinositol, the yeast Pichia pastoris as well as many other fungi have a second class, glucosylceramide, which has a glucose head group. These two sphingolipid classes are in addition distingu...