Imaging of Intact Tissue Sections: Moving beyond the Microscope

MALDI-imaging MS is a new molecular imaging technology for direct in situ analysis of thin tissue sections. Multiple analytes can be monitored simultaneously without prior knowledge of their identities and without the need for target-specific reagents such as antibodies. Imaging MS provides importan...

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Published inThe Journal of biological chemistry Vol. 286; no. 29; pp. 25459 - 25466
Main Authors Seeley, Erin H., Schwamborn, Kristina, Caprioli, Richard M.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 22.07.2011
American Society for Biochemistry and Molecular Biology
Subjects
Animals
Drugs
Humans
Imaging Mass Spectrometry
Lipids
Mass Spectrometry (MS)
Metabolites
Minireviews
Molecular Imaging - methods
Peptides
Peptides - metabolism
Pharmaceutical Preparations - metabolism
Proteins
Proteins - metabolism
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Tissue
Drugs
Proteins
Tissue
Peptides
Metabolites
Proteomics
Mass Spectrometry (MS)
Imaging Mass Spectrometry
Lipids
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Summary:MALDI-imaging MS is a new molecular imaging technology for direct in situ analysis of thin tissue sections. Multiple analytes can be monitored simultaneously without prior knowledge of their identities and without the need for target-specific reagents such as antibodies. Imaging MS provides important insights into biological processes because the native distributions of molecules are minimally disturbed, and histological features remain intact throughout the analysis. A wide variety of molecules can be imaged, including proteins, peptides, lipids, drugs, and metabolites. Several specific examples are presented to highlight the utility of the technology.
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Present address: Inst. of Pathology, Technical University Munich, Ismaningerstr. 22, 81675 Munich, Germany.
Both authors contributed equally to this work.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.R111.225854