Biases for detecting arbuscular mycorrhizal fungal mixture by terminal restriction fragment length polymorphism (T-RFLP)

• Published in: World journal of microbiology & biotechnology Vol. 30; no. 1; pp. 77 - 86
• Main Authors: Watanarojanaporn, N., Longtonglang, A., Boonkerd, N., Tittabutr, P., Lee, J., Teaumroong, N.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.01.2014; Springer Nature B.V
• Subjects: Abundance; Amplification; Analysis; Applied Microbiology; Bias; Bias (Epidemiology); Biochemistry; Biomedical and Life Sciences; Biotechnology; Chloroform; classification; clones; Cloning; Deoxyribonucleic acid; Diagnostic Errors; DNA; Environmental Engineering/Biotechnology; Flowers & plants; Fungi; Genes; genetics; Genomics; Life Sciences; methods; Microbial activity; microbial communities; Microbiology; Microorganisms; Molecular Typing; Molecular Typing - methods; Mycological Typing Techniques; Mycological Typing Techniques - methods; Mycorrhizae; Mycorrhizae - classification; Mycorrhizae - genetics; mycorrhizal fungi; Original Paper; Phenols; Plasmids; Polymerase chain reaction; Polymorphism; Polymorphism, Restriction Fragment Length; Purification; purification methods; Relative abundance; restriction fragment length polymorphism; ribosomal RNA; RNA, Ribosomal, 18S; RNA, Ribosomal, 18S - genetics; species diversity; Species richness; Studies; Terminals; vesicular arbuscular mycorrhizae; T-RFs peak area; Plant genomic DNA; AMF plasmid DNA; T-RFLP; Pairwise mixes

Terminal restriction fragment length polymorphism (T-RFLP) analysis of amplified ribosomal RNA genes is used for profiling microbial communities and sometimes for species richness and relative abundance estimation in environmental samples. However, the T-RFLP fingerprint may be subject to biases dur...