Binding Affinity and Specificity of Neuromyelitis Optica Autoantibodies to Aquaporin-4 M1/M23 Isoforms and Orthogonal Arrays

• Published in: The Journal of biological chemistry Vol. 286; no. 18; pp. 16516 - 16524
• Main Authors: Crane, Jonathan M., Lam, Chiwah, Rossi, Andrea, Gupta, Tripta, Bennett, Jeffrey L., Verkman, A.S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06.05.2011; American Society for Biochemistry and Molecular Biology
• Subjects: Antibodies; Antibodies, Monoclonal - immunology; Antibody Affinity; Antibody Specificity; AQP4; Aquaporin; Aquaporin 4 - genetics; Aquaporin 4 - immunology; Autoantibodies - genetics; Autoantibodies - immunology; Biophysics; Cell Line; Fluorescence; Humans; Immunoglobulin G - immunology; Membrane Biology; Microscopic Imaging; Mutation; Neuromyelitis Optica; Neuromyelitis Optica - genetics; Neuromyelitis Optica - immunology; NMO; Water Channel; NMO; Neuromyelitis Optica; Antibodies; Fluorescence; AQP4; Biophysics; Microscopic Imaging; Water Channel; Aquaporin

Autoantibodies against astrocyte water channel aquaporin-4 (AQP4) are highly specific for the neuroinflammatory disease neuromyelitis optica (NMO). We measured the binding of NMO autoantibodies to AQP4 in human astrocyte-derived U87MG cells expressing M1 and/or M23 AQP4, or M23 mutants that do not f...