Agarose Spot as a Comparative Method for in situ Analysis of Simultaneous Chemotactic Responses to Multiple Chemokines

• Published in: Scientific reports Vol. 7; no. 1; pp. 1075 - 11
• Main Authors: Ahmed, Mohaned, Basheer, Haneen A., Ayuso, Jose M., Ahmet, Djevdet, Mazzini, Marco, Patel, Roshan, Shnyder, Steven D., Vinader, Victoria, Afarinkia, Kamyar
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.04.2017; Nature Portfolio
• Subjects: 13/21; 13/31; 14/63; 631/154/1435; 631/1647/767/70; Cell Line, Tumor; Chemokines - metabolism; Chemotaxis; Culture Media; Cytological Techniques - methods; Epithelial Cells - drug effects; Epithelial Cells - physiology; Humanities and Social Sciences; Humans; multidisciplinary; Science; Science (multidisciplinary); Sepharose

We describe a novel protocol to quantitatively and simultaneously compare the chemotactic responses of cells towards different chemokines. In this protocol, droplets of agarose gel containing different chemokines are applied onto the surface of a Petri dish, and then immersed under culture medium in...