Cell-Type-Specific Gene Expression Profiling in Adult Mouse Brain Reveals Normal and Disease-State Signatures

• Published in: Cell reports (Cambridge) Vol. 26; no. 9; pp. 2477 - 2493.e9
• Main Authors: Merienne, Nicolas, Meunier, Cécile, Schneider, Anne, Seguin, Jonathan, Nair, Satish S., Rocher, Anne B., Le Gras, Stéphanie, Keime, Céline, Faull, Richard, Pellerin, Luc, Chatton, Jean-Yves, Neri, Christian, Merienne, Karine, Déglon, Nicole
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26.02.2019
• Subjects: Animals; Biochemistry, Molecular Biology; Brain - metabolism; cell-type-specific profiling; DNA - chemistry; Epigenesis, Genetic; epigenetics; Gene Expression Profiling - methods; Genomics; HD patients; HD transgenic mice; Humans; Huntington Disease - genetics; Huntington Disease - metabolism; Huntington’s disease; Laser Capture Microdissection - methods; Life Sciences; Male; Mice; Mice, Transgenic; MicroRNAs - metabolism; Neurobiology; Neurons and Cognition; Nucleic Acid Conformation; RNA, Messenger - metabolism; striatum; Transcription Factors - metabolism; transcriptomics; HD transgenic mice; striatum; transcriptomics; cell-type-specific profiling; HD patients; Huntington’s disease; epigenetics

The role of brain cell-type-specific functions and profiles in pathological and non-pathological contexts is still poorly defined. Such cell-type-specific gene expression profiles in solid, adult tissues would benefit from approaches that avoid cellular stress during isolation. Here, we developed su...