Attempt to Isolate Elephant Endotheliotropic Herpesvirus (EEHV) Using a Continuous Cell Culture System

• Published in: Animals (Basel) Vol. 10; no. 12; p. 2328
• Main Authors: Photichai, Kornravee, Guntawang, Thunyamas, Sittisak, Tidaratt, Kochagul, Varankpicha, Chuammitri, Phongsakorn, Thitaram, Chatchote, Thananchai, Hathairat, Chewonarin, Teera, Sringarm, Korawan, Pringproa, Kidsadagon
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.12.2020; MDPI
• Subjects: Cell culture; Colon; culture media; Deoxyribonucleic acid; DNA; DNA-directed DNA polymerase; elephant endotheliotropic herpesvirus; Elephantid betaherpesvirus 1; Elephas maximus; Fibroblasts; genes; Genetic testing; Genomes; heart; humans; immunohistochemistry; immunoperoxidase monolayer assay; in vitro; Infections; isolation; Kidneys; liver; lungs; myeloid leukemia; quantitative polymerase chain reaction; spleen; terminase; Viruses; United States > US; Germany; elephant endotheliotropic herpesvirus; isolation; cell culture; in vitro

Elephant endotheliotropic herpesvirus (EEHV) infection is known to cause acute fatal hemorrhagic disease, which has killed many young Asian elephants (Elephas maximus). Until recently, in vitro isolation and propagation of the virus have not been successful. This study aimed to isolate and propagate...