HPV in full thickness cervical biopsies: high prevalence in CIN 2 and CIN 3 detected by a sensitive PCR method

• Published in: The Journal of pathology Vol. 165; no. 4; p. 301
• Main Authors: Arends, M J, Donaldson, Y K, Duvall, E, Wyllie, A H, Bird, C C
• Format: Journal Article
• Language: English
• Published: England 01.12.1991
• Subjects: Adolescent; Adult; Base Sequence; Cervix Uteri - microbiology; Cervix Uteri - pathology; DNA, Viral - analysis; Female; Humans; Middle Aged; Molecular Sequence Data; Papillomaviridae - classification; Papillomaviridae - genetics; Papillomaviridae - isolation & purification; Polymerase Chain Reaction - methods; Uterine Cervical Neoplasms - microbiology; Uterine Cervical Neoplasms - pathology

A new type-specific, sensitive, non-radioactive assay is described for the detection of human papillomavirus (HPV) DNA in tissues. Sequences within the E6 gene were amplified by the polymerase chain reaction (PCR), using primer pairs which clearly distinguish HPV types, including those with close se...