Large-scale identification of proteins expressed in mouse embryonic stem cells

• Published in: Proteomics (Weinheim) Vol. 5; no. 5; pp. 1346 - 1361
• Main Authors: Nagano, Kohji, Taoka, Masato, Yamauchi, Yoshio, Itagaki, Chiharu, Shinkawa, Takashi, Nunomura, Kazuto, Okamura, Nobuko, Takahashi, Nobuhiro, Izumi, Tomonori, Isobe, Toshiaki
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.04.2005; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Biomarker; Cell Line; Embryo, Mammalian; Embryonic stem cell; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Gene ontology; Humans; Mass Spectrometry; Mice; Miscellaneous; Molecular Sequence Data; Protein Array Analysis; Protein Processing, Post-Translational; Proteins; Proteome; Proteome - analysis; Stem Cells - cytology; Stem Cells - physiology; Transcription Factors - analysis; Biomarker; Stem cell; Rodentia; Biological marker; Embryonic stem cell; Proteome; Identification; Protein; Gene ontology; Vertebrata; Mammalia; Gene; Mouse; Proteomics; Mass spectrometry

A protein subset expressed in the mouse embryonic stem (ES) cell line, E14‐1, was characterized by mass spectrometry‐based protein identification technology and data analysis. In total, 1790 proteins including 365 potential nuclear and 260 membrane proteins were identified from tryptic digests of to...