Active probing of the mechanical properties of biological and synthetic vesicles

• Published in: Biochimica et biophysica acta. General subjects Vol. 1865; no. 4; p. 129486
• Main Authors: Piontek, Melissa C., Lira, Rafael B., Roos, Wouter H.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2021
• Subjects: Atomic force microscopy (AFM); biophysics; drug carriers; Electrodeformation; electroporation; Mechanical properties; Micropipette aspiration (MPA); nanotechnology; optical traps; Optical tweezers (OT); Vesicles; EpCAM; FDC; AFM; Micropipette aspiration (MPA); FLIM; Vesicles; RBC; DHPE; DIC; PBS; Atomic force microscopy (AFM); AC; ESCRT; Syt1; Optical tweezers (OT); OT; Mechanical properties; GUV; LUV; Electrodeformation; EV; PEG; cw; PE; SUV; Cryo-EM; CHMP2B; MVB; ATP; DC; MPA
• ISSN: 0304-4165; 1872-8006; 1872-8006
• DOI: 10.1016/j.bbagen.2019.129486

The interest in mechanics of synthetic and biological vesicles has been continuously growing during the last decades. Liposomes serve as model systems for investigating fundamental membrane processes and properties. More recently, extracellular vesicles (EVs) have been investigated mechanically as well. EVs are widely studied in fundamental and applied sciences, but their material properties remained elusive until recently. Elucidating the mechanical properties of vesicles is essential to unveil the mechanisms behind a variety of biological processes, e.g. budding, vesiculation and cellular uptake mechanisms. The importance of mechanobiology for studies of vesicles and membranes is discussed, as well as the different available techniques to probe their mechanical properties. In particular, the mechanics of vesicles and membranes as obtained by nanoindentation, micropipette aspiration, optical tweezers, electrodeformation and electroporation experiments is addressed. EVs and liposomes possess an astonishing rich, diverse behavior. To better understand their properties, and for optimization of their applications in nanotechnology, an improved understanding of their mechanical properties is needed. Depending on the size of the vesicles and the specific scientific question, different techniques can be chosen for their mechanical characterization. Understanding the mechanical properties of vesicles is necessary to gain deeper insight in the fundamental biological mechanisms involved in vesicle generation and cellular uptake. This furthermore facilitates technological applications such as using vesicles as targeted drug delivery vehicles. Liposome studies provide insight into fundamental membrane processes and properties, whereas the role and functioning of EVs in biology and medicine are increasingly elucidated. [Display omitted] •The force response of vesicles reveals cell mechanics insights.•Liposomes serve as biomembrane models to scrutinize fundamental membrane processes.•Optical imaging techniques are versatile to reveal vesicle mechanical properties.•AFM is used to study the mechanical properties of submicrometer vesicles.•Vesicle mechanics can be described by tension, bending rigidity, membrane curvature.